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Titolo:
CONFOCAL LASER-SCANNING MICROSCOPY FOR DETERMINING THE STRUCTURE OF AND KERATINOCYTE INFILTRATION THROUGH COLLAGEN SPONGES
Autore:
HANTHAMRONGWIT M; WILKINSON R; OSBORNE C; REID WH; GRANT MH;
Indirizzi:
UNIV STRATHCLYDE,BIOENGN UNIT,WOLFSON CTR,106 ROTTENROW GLASGOW G4 0NW LANARK SCOTLAND UNIV STRATHCLYDE,BIOENGN UNIT,WOLFSON CTR GLASGOW G4 0NW LANARK SCOTLAND GLASGOW ROYAL INFIRM,BURNS UNIT GLASGOW G4 0SF LANARK SCOTLAND
Titolo Testata:
Journal of biomedical materials research
fascicolo: 3, volume: 30, anno: 1996,
pagine: 331 - 339
SICI:
0021-9304(1996)30:3<331:CLMFDT>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
EXTRACELLULAR-MATRIX; HYALURONIC-ACID; FIBROBLASTS; DIFFERENTIATION; FIBRONECTIN; SUBSTITUTE; SUBSTRATE; GROWTH; MODEL; SKIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
12
Recensione:
Indirizzi per estratti:
Citazione:
M. Hanthamrongwit et al., "CONFOCAL LASER-SCANNING MICROSCOPY FOR DETERMINING THE STRUCTURE OF AND KERATINOCYTE INFILTRATION THROUGH COLLAGEN SPONGES", Journal of biomedical materials research, 30(3), 1996, pp. 331-339

Abstract

The development of artificial skin substitutes based on cultured cells and biomaterials such as collagen requires an understanding of cellular interactions with the substrate. In this study, human keratinocytes were cultured on the surface of collagen sponges, and confocal laser-scanning microscopy (CLSM) was used to assess both the microstructureof the sponge, and the cell morphology and distribution throughout the sponge. It was found that the pore size increased with increasing depth into the sponge. Both pore size and fiber thickness increased during incubation for up to 10 days at 37 degrees C in culture medium in the absence of cells. This latter effect was not observed when the sponges were incubated in distilled water. Keratinocytes penetrated into the sponge even after only 3 days in culture. By 10 days in culture, the cells had penetrated to the maximum depth that could be examined (120 mu m from the sponge surface). In the presence of cells, the inner structure of the collagen sponge had altered after 10 days in culture, with the collagen fibers becoming thicker, and pore geometry less regular. The mechanism responsible for this is unknown at present. Although the presence of the keratinocytes increases distortion of the spongestructure, factors from the medium itself also contribute to this effect. CLSM is a powerful tool for assessing cellular interactions with bioimplants, providing both qualitative and quantitative information. It offers many advantages over scanning electron microscopy (SEM) and histological techniques. CLSM minimizes the time-consuming, extensive preparation of samples required with the latter two methods, and allows noninvasive serial optical sectioning of intact samples. (C) 1996 John Wiley & Sons, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/09/20 alle ore 16:10:05