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Titolo:
INFLUENCE OF RETINOIC ACID ON THE EXPRESSION OF CYTOKERATINS, VIMENTIN AND ICAM-1 IN HUMAN GINGIVAL EPITHELIA IN-VITRO
Autore:
GAO Z; MACKENZIE IC;
Indirizzi:
UNIV TEXAS,HLTH SCI CTR,DENT SCI INST,DENT BRANCH 4109,POB 20068 HOUSTON TX 77225 UNIV TEXAS,HLTH SCI CTR,DENT SCI INST,DENT BRANCH 4109 HOUSTON TX 77225
Titolo Testata:
Journal of Periodontal Research
fascicolo: 2, volume: 31, anno: 1996,
pagine: 81 - 89
SICI:
0022-3484(1996)31:2<81:IORAOT>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
JUNCTIONAL EPITHELIUM; TRANSCRIPTIONAL REGULATION; GENE-EXPRESSION; VITAMIN-A; DIFFERENTIATION; RECEPTORS; CELLS; MOUSE; KERATINOCYTES; REGENERATION;
Keywords:
GINGIVA; EPITHELIA; RETINOIDS; CELL CULTURE; DIFFERENTIATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
44
Recensione:
Indirizzi per estratti:
Citazione:
Z. Gao e I.C. Mackenzie, "INFLUENCE OF RETINOIC ACID ON THE EXPRESSION OF CYTOKERATINS, VIMENTIN AND ICAM-1 IN HUMAN GINGIVAL EPITHELIA IN-VITRO", Journal of Periodontal Research, 31(2), 1996, pp. 81-89

Abstract

Phenotypic differences exist in vivo between junctional (JE) and oralgingival (OGE) epithelia and an in vitro system has been developed that maintains phenotypic differences. This system, which permits in vitro studies of factors that may influence the epithelial phenotype, wasused to investigate the effects of retinoic acid (RA) on epithelial expression of various markers known to distinguish JE from OGE. Primarycultures of JE and OGE were initiated from defined gingival regions and were subcultured and grown for 48 h in 96-well plates or on multiple-well slides. Control cultures were grown in medium supplemented withdelipidized serum and all-trans RA was added to experimental groups. Other cultures were grown in a defined RA-free medium. Cultures were examined using monoclonal antibodies against cytokeratins, vimentin, and ICAM-1 and binding displayed by indirect immunocytochemical staining. Staining reactions were assessed by direct microscopic observation and assayed by spectrophotometric quantitation. The results showed thatRA had minor effects on the marker expression of JE but markedly enhanced expression of cytokeratins 8, 18, 19, vimentin and ICAM-1 in OGE. These markers, which normally distinguish JE from OGE, were expressedat levels approaching or exceeding those of control JE cultures. These observations indicate that RA responsive mechanisms affect the phenotypes expressed by epithelia in vitro and suggest that such mechanismsmay be related to the different phenotypic patterns expressed by gingival epithelia in vivo.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/09/20 alle ore 06:36:56