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Titolo:
A ROLE FOR COFACTORS IN SYNERGISTIC AND CELL-SPECIFIC ACTIVATION BY RETINOIC ACID RECEPTORS AND RETINOID-X RECEPTOR
Autore:
FOLKERS GE; VANDERBURG B; VANDERSAAG PT;
Indirizzi:
NETHERLANDS INST DEV BIOL,HUBRECHT LAB,UPPSALALAAN 8 3584 CT UTRECHT NETHERLANDS NETHERLANDS INST DEV BIOL,HUBRECHT LAB 3584 CT UTRECHT NETHERLANDS
Titolo Testata:
Journal of steroid biochemistry and molecular biology
fascicolo: 1-6, volume: 56, anno: 1996,
pagine: 119 - 129
SICI:
0960-0760(1996)56:1-6<119:ARFCIS>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
EUKARYOTIC TRANSCRIPTIONAL ACTIVATORS; STEROID-HORMONE RECEPTORS; ADENOVIRUS E1A PROTEIN; ESTROGEN-RECEPTOR; RESPONSE ELEMENT; BETA-GENE; MULTIPLE; ISOFORMS; DOMAINS; MEDIATE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
62
Recensione:
Indirizzi per estratti:
Citazione:
G.E. Folkers et al., "A ROLE FOR COFACTORS IN SYNERGISTIC AND CELL-SPECIFIC ACTIVATION BY RETINOIC ACID RECEPTORS AND RETINOID-X RECEPTOR", Journal of steroid biochemistry and molecular biology, 56(1-6), 1996, pp. 119-129

Abstract

Transcriptional activation is thought to be mediated by DNA-bound activators through interaction with a basal transcription factor thereby stabilizing the pre-initiation complex. For such interaction cofactorssuch as TAFs, bridging proteins, mediators or intermediary proteins are required by binding simultaneously to the activator and the target. We have investigated the activation functions (AFs) of both RAR beta and RXR alpha and show that both activators contain two homologous AFs. By comparing the capacity to activate transcription by these AFs on several promoters, both as full-length receptors and as fusion-proteins of AFs with the DNA-binding domain of the yeast transcription factorGAL-4, we were able to show that these AFs function by different mechanisms. We found that the activity of these AFs is cell-type specific,as they are more active in certain cell lines than in others. Furthermore we observed that the AFs of RAR beta and RXR alpha can activate transcription synergistically both as GAL-fusion protein and with full-length receptors. For AF-2 of RAR beta we observed cell type-dependentdifferences in synergistic activation and we show that the E1A protein, which functions as a cofactor for RAR beta, permits synergistic activation in cell lines in which in the absence of this cofactor transcription occurs non-synergistically. We propose a model in which severalnon cell type specific cofactors and cell-specific cofactors act together to form a more stable pre-initiation complex explaining the observed cell-specific synergistic activation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/11/20 alle ore 19:12:27