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Titolo:
EXPRESSION OF FGFR2 BEK AND K-SAM MESSENGER-RNA VARIANTS IN NORMAL AND MALIGNANT HUMAN BREAST
Autore:
LUQMANI YA; BANSAL GS; MORTIMER C; BULUWELA L; COOMBES RC;
Indirizzi:
KUWAIT UNIV,FAC ALLIED HLTH,POB 31470 SALAIBIKHAT 90805 KUWAIT CHARING CROSS HOSP,DEPT MED ONCOL LONDON W6 8RF ENGLAND
Titolo Testata:
European journal of cancer
fascicolo: 3, volume: 32A, anno: 1996,
pagine: 518 - 524
SICI:
0959-8049(1996)32A:3<518:EOFBAK>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
FIBROBLAST GROWTH-FACTOR; LIGAND-BINDING SPECIFICITY; FACTOR RECEPTOR GENES; MESSENGER-RNA; CANCER; ISOFORMS; TISSUE; DOMAIN;
Keywords:
HUMAN BREAST; FIBROBLAST GROWTH FACTOR RECEPTOR; BEK; K-SAM; PCR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
28
Recensione:
Indirizzi per estratti:
Citazione:
Y.A. Luqmani et al., "EXPRESSION OF FGFR2 BEK AND K-SAM MESSENGER-RNA VARIANTS IN NORMAL AND MALIGNANT HUMAN BREAST", European journal of cancer, 32A(3), 1996, pp. 518-524

Abstract

The expression of mRNA encoding alternative forms of fibroblast growth factor receptor 2 (FGFR2) differing in the carboxy terminal half of their third immunoglobulin-like domain, was investigated in 77 human breast cancer tissues, 12 non-malignant breast biopsies and 29 cell lines, using a reverse transcriptase (RT) polymerase chain reaction (PCR)method. RNA from the two tissue groups yielded PCR product corresponding to both the BEK and the K-SAM form; amounts normalised to glyceraldehyde phosphate dehydrogenase product were similar in both groups. The level of either variant or of the total FGFR2 product was essentially unrelated to prognosis or clinical status except that patients with advanced clinical T staging had a higher proportion of BEK to K-SAM (P= 0.01). RNA from 1/2 normal breast derived and 8/10 breast cancer cell lines expressed exclusively or predominantly the K-SAM form; 2/10 had significant amounts of both BEK and K-SAM mRNA. Of 12 other epithelial lines, seven expressed mainly K-SAM mRNA, four expressed BEK and one was negative. Of five non-epithelial lines, one was negative, two expressed only BEK mRNA and two had significant amounts of both variants. We conclude that tissue levels of FGFR2 mRNA are unaltered in breast cancer extracts and that the splicing mechanism for this exon selection appears not to be significantly disrupted.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/11/20 alle ore 04:39:12