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Titolo:
EFFECTS OF SYNTHETIC PEPTIDO-LEUKOTRIENES ON BONE-RESORPTION IN-VITRO
Autore:
GARCIA C; QIAO M; CHEN D; KIRCHEN M; GALLWITZ W; MUNDY GR; BONEWALD LF;
Indirizzi:
UNIV TEXAS,HLTH SCI CTR,DEPT MED ENDOCRINOL,7703 FLOYD CURL DR SAN ANTONIO TX 78284 UNIV TEXAS,HLTH SCI CTR,DEPT MED ENDOCRINOL SAN ANTONIO TX 78284 ORTHOPED HOSP,ORTHOPAED RES CTR LOS ANGELES CA 90007
Titolo Testata:
Journal of bone and mineral research
fascicolo: 4, volume: 11, anno: 1996,
pagine: 521 - 529
SICI:
0884-0431(1996)11:4<521:EOSPOB>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
GUINEA-PIG LUNG; GROWTH-FACTOR-BETA; SIGNAL TRANSDUCTION; ARACHIDONIC-ACID; BINDING-AFFINITY; D4 RECEPTORS; CELLS; IDENTIFICATION; OSTEOCLASTS; METABOLISM;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
52
Recensione:
Indirizzi per estratti:
Citazione:
C. Garcia et al., "EFFECTS OF SYNTHETIC PEPTIDO-LEUKOTRIENES ON BONE-RESORPTION IN-VITRO", Journal of bone and mineral research, 11(4), 1996, pp. 521-529

Abstract

Peptido-leukotrienes are short-lived organic molecules known to have potent biological effects as mediators of inflammation, hypersensitivity and respiratory disorders, However, little is known concerning their effects on bone cells, We have shown previously that stromal cells isolated from a human giant cell tumor secrete 5-HETE (5-hydroxyeicosatetraenoic acid) and the peptido-leukotrienes, also known as the cysteinyl leukotrienes LTC(4), LTD(4), and LTE(4). These eicosanoids,were shown to stimulate the multinucleated giant cells obtained from these tumors to form resorption lacunae on sperm whale dentine, Here, we show that the peptido-leukotrienes also stimulate isolated avian osteoclast-like cells to form resorption lacunae and to increase their content of tartrate-resistant acid phosphatase. LTD(4) increased Ca-45 release from murine calvarial bone organ cultures, but not from fetal rat longbone cultures, Isolated avian osteoclast-like cells were chosen to perform receptor binding studies, as this population is the most homogenous source of osteoclasts available. After the precursors had fused toform multinucleated cells, receptor binding assays were performed, Scatchard analysis of saturation binding data showed a single class of binding sites, with a dissociation constant (K-d) of 0.53 nM and a receptor density of 5,200 receptors per cell, Competition binding studies showed receptor specificity using a specific LTD, receptor antagonist ZM 198,615, These data show that the peptido-leukotrienes activate highly enriched populations of isolated avian osteoclast-like cells, and also that specific LTD(4) receptors are present in this cell population.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/01/20 alle ore 19:36:03