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Titolo:
A FAMILY OF GENES-CODING FOR 2 SEROLOGICALLY DISTINCT CHICKEN INTERFERONS
Autore:
SICK C; SCHULTZ U; STAEHELI P;
Indirizzi:
UNIV FREIBURG,DEPT VIROL,INST MED MIKROBIOL & HYG,HERMANN HERDWER STR11 D-79008 FREIBURG GERMANY UNIV FREIBURG,DEPT VIROL,INST MED MIKROBIOL & HYG D-79008 FREIBURG GERMANY
Titolo Testata:
The Journal of biological chemistry
fascicolo: 13, volume: 271, anno: 1996,
pagine: 7635 - 7639
SICI:
0021-9258(1996)271:13<7635:AFOGF2>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
MACROPHAGE ACTIVATION; GAMMA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
16
Recensione:
Indirizzi per estratti:
Citazione:
C. Sick et al., "A FAMILY OF GENES-CODING FOR 2 SEROLOGICALLY DISTINCT CHICKEN INTERFERONS", The Journal of biological chemistry, 271(13), 1996, pp. 7635-7639

Abstract

Southern blot analysis and screening of a genomic lambda phage library with the previously cloned chicken interferon (IFN) cDNA indicated that the chicken genome contains at least 10 IFN genes, A particularly strongly hybridizing phage clone that we analyzed in more detail carried a head to tail arrangement of three intron-less IFN genes that differed from each other and from the cloned chicken IFN cDNA by only a few base changes, The primary translation products of these three IFN genes consist of 193 amino acids, and the mature proteins are composed of 162 amino acids. All three genes of this IFN family, designated IFN1, yielded active chicken IFN when expressed individually in transfected COS7 cells. A weakly hybridizing phage clone contained an additionalintron-less chicken IFN gene, designated IFN2, whose product was 57% identical to chicken IFN1. Southern blot analysis suggested that the chicken genome contains a single IFN2 gene, The primary translation product of IFN2 consists of 203 amino acids, and the mature protein is composed of 176 amino acids, Purified recombinant chicken IFN2 from Escherichia coli had a specific antiviral activity of about 10(6) units/mg, which was about 20-fold lower than that of chicken IFN1 purified in parallel, The antiviral activity of chicken IFN2 from E. coli or from transfected COS7 cells could not be neutralized by antiserum to recombinant chicken IFN1, Thus, like mammals, the chicken has a large numberof type I IFN genes that code for at least two serologically distinctantiviral activities.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/01/20 alle ore 09:31:27