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Titolo:
IMPROVED LOCALIZATION OF FLUORESCENT TYRAMIDES FOR FLUORESCENCE IN-SITU HYBRIDIZATION USING DEXTRAN SULFATE AND POLYVINYL-ALCOHOL
Autore:
VANGIJLSWIJK RPM; WIEGANT J; RAAP AK; TANKE HJ;
Indirizzi:
LEIDEN STATE UNIV,DEPT CYTOCHEM & CYTOMETRY,WASSENAARSEWEG 72 2333 ALLEIDEN NETHERLANDS
Titolo Testata:
The Journal of histochemistry and cytochemistry
fascicolo: 4, volume: 44, anno: 1996,
pagine: 389 - 392
SICI:
0022-1554(1996)44:4<389:ILOFTF>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Keywords:
FLUORESCENT IN SITU HYBRIDIZATION; CARD; LOCALIZATION; DEXTRAN SULFATE; POLYVINYL ALCOHOL;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
6
Recensione:
Indirizzi per estratti:
Citazione:
R.P.M. Vangijlswijk et al., "IMPROVED LOCALIZATION OF FLUORESCENT TYRAMIDES FOR FLUORESCENCE IN-SITU HYBRIDIZATION USING DEXTRAN SULFATE AND POLYVINYL-ALCOHOL", The Journal of histochemistry and cytochemistry, 44(4), 1996, pp. 389-392

Abstract

Recently, a peroxidase-mediated amplification system has been described for immunofluorescence and fluorescence in situ hybridization studies. It is based on the deposition of hapten- or fluorochrome-labeled tyramide molecules. Although providing a significantly increased detection sensitivity compared to conventional procedures, its localization properties are inferior because of free diffusion of intermediate reaction products before they are immobilized. In enzyme cytochemistry, itis well established that improved localization of enzyme activity canbe achieved through the addition of viscosity-increasing polymers to the incubation media. In this study we analyzed the effect of different polymers on the localization sharpness and sensitivity of the tyramide-peroxidase reaction in FISH applications. Significantly improved localization of the fluorescent endproduct was observed using dextran sulfate or polyvinylalcohol (PVA) with, respectively, no or little loss of sensitivity.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 14:49:14