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Titolo:
BIOMARKER ALTERATIONS PRODUCED IN RAT LUNG BY INTRATRACHEAL INSTILLATIONS OF AIR PARTICULATE EXTRACTS AND CHEMOPREVENTION WITH ORAL N-ACETYLCYSTEINE
Autore:
IZZOTTI A; CAMOIRANO A; DAGOSTINI F; SCIACCA S; PAPA FD; CESARONE CF; DEFLORA S;
Indirizzi:
UNIV GENOA,INST HYG & PREVENT MED,VIA PASTORE 1 I-16132 GENOA ITALY UNIV GENOA,INST HYG & PREVENT MED I-16132 GENOA ITALY UNIV CATANIA,INST HYG & PREVENT MED I-95124 CATANIA ITALY UNIV GENOA,INST GEN PHYSIOL I-16132 GENOA ITALY
Titolo Testata:
Cancer research
fascicolo: 7, volume: 56, anno: 1996,
pagine: 1533 - 1538
SICI:
0008-5472(1996)56:7<1533:BAPIRL>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLYCYCLIC AROMATIC-HYDROCARBONS; PULMONARY ALVEOLAR MACROPHAGES; DIVERSE DNA ADDUCTS; POLY(ADP-RIBOSE) POLYMERASE; CIGARETTE-SMOKE; ASSAY; LIVER; BENZO)A>PYRENE; MUTAGENICITY; ENHANCEMENT;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
46
Recensione:
Indirizzi per estratti:
Citazione:
A. Izzotti et al., "BIOMARKER ALTERATIONS PRODUCED IN RAT LUNG BY INTRATRACHEAL INSTILLATIONS OF AIR PARTICULATE EXTRACTS AND CHEMOPREVENTION WITH ORAL N-ACETYLCYSTEINE", Cancer research, 56(7), 1996, pp. 1533-1538

Abstract

Organic matter extracts were obtained from particulates recovered from 10,000-m(3) air samples collected in Sicily (Italy). The overall concentrations of acenaphthene, benzo(a)pyrene, phenanthrene, anthracene,fluoranthene, and pyrene were 526 ng/m(3) air in a highly polluted urban area and 48 ng/m(3) in a rural area affected by motor vehicle traffic pollution, After metabolic activation, both samples were mutagenicin Salmonella typhimurium his strains of the TA and YG series, with potencies in TA100 of 140.7 and 11.8 revertants/m(3) air, respectively. The samples, resuspended in tricaprylin, were instilled intratracheally in Sprague-Dawley rats for 5 consecutive days, accounting for a cumulative dose in each animal of the organic fractions extracted from 400 m(3) air, which corresponds approximately to the volume of air inhaled by a man in 1 month. Treatment with the rural area sample and, at higher levels, with the urban area sample resulted in the formation of adducts to lung DNA, as assessed both by synchronous fluorescence spectrophotometry and by P-32 postlabeling, which showed the appearance ofup to six individual adducts emerging from diffuse diagonal radioactive zones. The adducts mere more efficiently detected by extraction with butanol than by digestion with nuclease P1. DNA binding of air particulate extracts was followed by alterations of early damage biomarkersonly in the rats treated with the urban area sample. Repair of DNA damage in lung cells was inferred from a significant stimulation of the nuclear enzyme poly(ADP-ribose) polymerase compared with that in sham-exposed rats. Among the cells recovered by bronchoalveolar lavage, an increase in polymorphonucleate leukocytes and cells of the ciliated respiratory epithelium was accompanied by a relative decrease in pulmonary alveolar macrophages. The frequency of micronuclei was significantly enhanced both in epithelial cells and alveolar macrophages, and binucleated macrophages were also more frequent in treated rats. The thiolN-acetylcysteine, one of the most promising cancer chemopreventive agents, was administered with drinking water to a group of animals receiving the air particulate polycyclic aromatic hydrocarbon fraction fromthe urban area. N-acetylcysteine prevented or considerably attenuatedthe alterations of all monitored parameters. These findings provide evidence that, even under outstandingly high exposure conditions, it ispossible to protect the respiratory tract from DNA-binding and DNA-damaging air particulate carcinogens.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/09/20 alle ore 05:29:40