Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
XENOBIOTIC METABOLISM ENZYME GENE-EXPRESSION IN HUMAN BRONCHIAL EPITHELIAL AND ALVEOLAR MACROPHAGE CELLS
Autore:
WILLEY JC; COY E; BROLLY C; UTELL MJ; FRAMPTON MW; HAMMERSLEY E; THILLY WG; OLSON D; CAIRNS K;
Indirizzi:
MED COLL OHIO,DEPT MED,DIV PULM & CRIT CARE MED,3000 ARLINGTON AVE TOLEDO OH 43699 MED COLL OHIO,DEPT PHYSIOL & MOLEC MED TOLEDO OH 43699 UNIV ROCHESTER,SCH MED,DEPT MED ROCHESTER NY 00000 UNIV ROCHESTER,SCH MED,DEPT ENVIRONM MED ROCHESTER NY 00000 MIT,CTR ENVIRONM HLTH SCI,DEPT TOXICOL BOSTON MA 00000
Titolo Testata:
American journal of respiratory cell and molecular biology
fascicolo: 3, volume: 14, anno: 1996,
pagine: 262 - 271
SICI:
1044-1549(1996)14:3<262:XMEGIH>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
CDNA-DIRECTED EXPRESSION; POLYMERASE CHAIN-REACTION; COMPLEMENTARY-DNA; HUMAN-LIVER; CHROMOSOMAL LOCALIZATION; EPOXIDE HYDROLASE; RISK ASSESSMENT; MESSENGER-RNAS; COS-1 CELLS; HUMAN-LUNG;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
53
Recensione:
Indirizzi per estratti:
Citazione:
J.C. Willey et al., "XENOBIOTIC METABOLISM ENZYME GENE-EXPRESSION IN HUMAN BRONCHIAL EPITHELIAL AND ALVEOLAR MACROPHAGE CELLS", American journal of respiratory cell and molecular biology, 14(3), 1996, pp. 262-271

Abstract

Human bronchial epithelial cells (BEC), a primary defense against inhaled materials, are the progenitor cells for bronchogenic carcinomas and have important metabolic capabilities. We used reverse transcriptase-polymerase chain reaction (RT-PCR) to identify xenobiotic metabolismenzymes expressed in primary BEC and alveolar macrophages (AM) of non-smoking volunteers. Cytochromes P450 (CYP) 1A1, 1B1, 2B7, 2E1, and 4B1 and microsomal epoxide hydrolase (mEH) were expressed in BEC but notAM. CYP2F1 was expressed in BEC, but it was expressed at barely detectable levels or not at all in AM. NADPH oxidoreductase (NADPH OR), microsomal glutathione transferase (GST 12), glutathione transferase mu, phenol sulfouansferase (PST), thermolabile phenol sulfotransferase (TLPST), and the clara cell-specific gene, CC10 were expressed in both BEC and AM. CYP3A4 and glucuronosyl transferases-1 and 2 were not expressed in either BEC or AM. In contrast to primary BEC, of the genes evaluated, the immortalized human bronchial epithelial cell line BEP2D constitutively expressed only CYP1A1, CYP2E1, NADPH OR, glucuronosyl transferase 1, GST 12, GST mu, PST, TL PST, and CC10. The loss of xenobiotic metabolism enzyme gene expression in the BEP2D cell line may result from either reduced exposure to inducing agents, or loss of differentiative characteristics in culture. It is clear from the data comparing BEC and AM that there are important intertissue differences in expression of xenobiotic metabolism enzymes.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 13:38:21