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Titolo:
RELEASE OF [H-3] NORADRENALINE FROM RAT HIPPOCAMPAL SYNAPTOSOMES BY NICOTINE - MEDIATION BY DIFFERENT NICOTINICRECEPTOR SUBTYPES FROM STRIATAL [H-3] DOPAMINE RELEASE
Autore:
CLARKE PBS; REUBEN M;
Indirizzi:
MCGILL UNIV,DEPT PHARMACOL & THERAPEUT,3655 DRUMMOND ST MONTREAL PQ H3G 1Y6 CANADA
Titolo Testata:
British Journal of Pharmacology
fascicolo: 4, volume: 117, anno: 1996,
pagine: 595 - 606
SICI:
0007-1188(1996)117:4<595:RO[NFR>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
CENTRAL-NERVOUS-SYSTEM; H-3 DOPAMINE RELEASE; ACETYLCHOLINE-RECEPTOR SUBUNIT; BINDING-SITES; ALPHA-BUNGAROTOXIN; PHARMACOLOGICAL CHARACTERIZATION; )H-3>-LABELED ACETYLCHOLINE; DISCRIMINATIVE STIMULUS; NOREPINEPHRINE RELEASE; NEURONAL BUNGAROTOXIN;
Keywords:
NICOTINE; ACETYLCHOLINE; DOPAMINE; NORADRENALINE; NICOTINIC RECEPTORS; SYNAPTOSOMES; STRIATUM; HIPPOCAMPUS; CHLORISONDAMINE; DIHYDRO-BETA-ERYTHROIDINE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
89
Recensione:
Indirizzi per estratti:
Citazione:
P.B.S. Clarke e M. Reuben, "RELEASE OF [H-3] NORADRENALINE FROM RAT HIPPOCAMPAL SYNAPTOSOMES BY NICOTINE - MEDIATION BY DIFFERENT NICOTINICRECEPTOR SUBTYPES FROM STRIATAL [H-3] DOPAMINE RELEASE", British Journal of Pharmacology, 117(4), 1996, pp. 595-606

Abstract

1 The aim of the present experiment was to characterize nicotine-evoked [H-3]-noradrenaline ([H-3]-NA) release from rat superfused hippocampal synaptosomes, using striatal [H-3]-dopamine release for comparison. 2 (-)-Nicotine, cytisine, DMPP and acetylcholine (ACh) (with esterase inhibitor and muscarinic receptor blocker) increased NA release in aconcentration-dependent manner (EC(50) 6.5 mu M, 8.2 mu M, 9.3 mu M, and 27 mu M, respectively) with similar efficacy. 3 Nicotine released striatal dopamine more potently than hippocampal NA (EC(50) 0.16 mu M VS. 6.5 mu M). (+)-Anatoxin-a also increased dopamine more potently than NA (EC(50) 0.05 mu M VS 0.39 mu M), and maximal effects were similar to those of nicotine. Isoarecolone (10-320 mu M) released dopamine more effectively than NA but a maximal effect was not reached. (-)-Lobeline (10-320 mu M) evoked dopamine release, but the effect was large and delayed with respect to nicotine; NA release was not increased but rather depressed at high concentrations of lobeline. High K+ (10 mM) released dopamine and NA to similar extents. 4 Addition of the 5-hydroxytryptamine (5-HT) reuptake blocker, citalopram (1 mu M) to hippocampal synaptosomes affected neither basal NA release nor nicotine-evoked release. 5 The nicotinic antagonist, mecamylamine (10 mu M), virtually abolished NA and dopamine release evoked by high concentrations of nicotine, ACh, cytisine, isoarecolone, and anatoxin-a. Although NA release evoked by DMPP (100 mu M) was entirely mecamylamine-sensitive, DMPP-evoked dopamine release was only partially blocked. Dopamine release evoked by lobeline (320 mu M) was completely mecamylamine-insensitive. 6 The nicotinic antagonists dihydro-beta-erythroidine and methyllycaconitine inhibited nicotine-evoked dopamine release approximately 30 fold more potently than NA release. In contrast, the antagonist chlorisondamine, displayed a reverse sensitivity, whereas trimetaphan and mecamylamine did not preferentially block either response. None of these antagonists, given at a high concentration, significantly altered release evoked by high K+. 7 Blockade of nicotine-evoked transmitter releaseby methyllycaconitine and dihydro-beta-erythroidine was surmounted bya high concentration of nicotine (100 mu M), but blockade by mecamylamine, chlorisondamine, and trimetaphan was insurmountable. 8 Nicotine-evoked NA release was unaffected by tetrodotoxin, whereas veratridine-evoked NA release was virtually abolished. 9 We conclude that presynaptic nicotinic receptors associated with striatal dopamine and hippocampal NA. terminals differ pharmacologically. In situ hybridization studies suggest that nigrostriatal dopaminergic neurones express mainly alpha 4, alpha 5, and beta 2 nicotinic cholinoceptor subunits, whereas hippocampal-projecting noradrenaline (NA) neurones express alpha 3, beta 2 and beta 4 subunits. Pharmacological comparisons of recombinant receptors suggest that release of hippocampal NA may be modulated by receptors containing alpha 3 and beta 4 subunits.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 12/07/20 alle ore 06:51:30