Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
INVOLVEMENT OF PLATELET CYCLIC-GMP BUT NOT CYCLIC-AMP SUPPRESSION IN LEUKOCYTE-DEPENDENT PLATELET-ADHESION TO ENDOTHELIAL-CELLS INDUCED BY PLATELET-ACTIVATING-FACTOR IN-VITRO
Autore:
HIRAFUJI M; NEZU A; SHINODA H; MINAMI M;
Indirizzi:
HLTH SCI UNIV HOKKAIDO,FAC PHARMACEUT SCI,DEPT PHARMACOL ISHIKARI HOKKAIDO 06102 JAPAN TOHOKU UNIV,SCH DENT,DEPT PHARMACOL SENDAI MIYAGI 980 JAPAN
Titolo Testata:
British Journal of Pharmacology
fascicolo: 2, volume: 117, anno: 1996,
pagine: 299 - 304
SICI:
0007-1188(1996)117:2<299:IOPCBN>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
RELAXING FACTOR; NITRIC-OXIDE; VESSEL WALL; DIFFERENTIAL INHIBITION; VASCULAR ENDOTHELIUM; FACTOR INVITRO; L-ARGININE; AGGREGATION; BOVINE; PHOSPHODIESTERASE;
Keywords:
PLATELET ADHESION; ENDOTHELIAL CELLS; LEUKOCYTES; EDRF; CYCLIC AMP; CYCLIC GMP; PLATELET-ACTIVATING FACTOR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
34
Recensione:
Indirizzi per estratti:
Citazione:
M. Hirafuji et al., "INVOLVEMENT OF PLATELET CYCLIC-GMP BUT NOT CYCLIC-AMP SUPPRESSION IN LEUKOCYTE-DEPENDENT PLATELET-ADHESION TO ENDOTHELIAL-CELLS INDUCED BY PLATELET-ACTIVATING-FACTOR IN-VITRO", British Journal of Pharmacology, 117(2), 1996, pp. 299-304

Abstract

1 Incubation of endothelial cells with platelets in the absence or the presence of PAF (10 nM) markedly increased platelet cyclic AMP levels, which were significantly decreased by indomethacin (3 mu M). Coincubation of endothelial cells and platelets with polymorphonuclear leukocytes (PMNs) did not change the platelet cyclic AMP levels. 2 Incubation of endothelial cells with platelets in the absence of PAF increasedplatelet cyclic GMP levels, which were increased 3.5 fold by PAF. These cyclic CTMP levels were significantly decreased by N-G-nitro-L-arginine (100 mu M), and completely by methylene blue (10 mu M). When endothelial cells and platelets were co-incubated with PMNs, the cyclic GMP level in the cell mixture was 42.5 and 65.3% lower than that in endothelial cells and platelets without and with PAF stimulation, respectively. 3 PAF induced platelet adhesion to endothelial cells only when PMNs were present. Methylene blue dose-dependently potentiated the PMN-dependent platelet adhesion induced by PAF, although it had no effect in the absence of PMNs. 4 Sodium nitroprusside and 8-bromo cyclic GMP but not dibutyryl cyclic AMP significantly, although partially, inhibited the platelet adhesion. Inhibition of cyclic GMP-specific phosphodiesterase by zaprinast slightly inhibited the PMN-induced platelet adhesion and potentiated the inhibitory effect of 8-bromo cyclic GMP, while these drugs markedly inhibited the adhesion of platelet aggregates induced by PMN sonicates. 5 These results suggest that the impairment by activated PMNs of EDRF-induced platelet cyclic GMP formation is involved in part in the mechanism of PMN-dependent platelet adhesion to endothelial cells induced by PAF in vitro. The precise mechanism still remains to be clarified.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 31/03/20 alle ore 05:09:15