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Titolo:
EFFECT OF 3,4-DIAMINOPYRIDINE ON RAT EXTENSOR DIGITORUM LONGUS MUSCLEPARALYZED BY LOCAL INJECTION OF BOTULINUM NEUROTOXIN
Autore:
ADLER M; MACDONALD DA; SELLIN LC; PARKER GW;
Indirizzi:
USA,MED RES INST CHEM DEF,DIV PATHOPHYSIOL,NEUROTOXICOL BRANCH ABERDEEN PROVING GROUND MD 21010 USA,MED RES INST INFECT DIS,DIV TOXINOL FREDERICK MD 21701
Titolo Testata:
Toxicon
fascicolo: 2, volume: 34, anno: 1996,
pagine: 237 - 249
SICI:
0041-0101(1996)34:2<237:EO3ORE>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
NEUROMUSCULAR-JUNCTION; TETANUS TOXIN; NEUROTRANSMITTER RELEASE; SYNAPTOBREVIN; PROTEASE; SNAP-25;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
40
Recensione:
Indirizzi per estratti:
Citazione:
M. Adler et al., "EFFECT OF 3,4-DIAMINOPYRIDINE ON RAT EXTENSOR DIGITORUM LONGUS MUSCLEPARALYZED BY LOCAL INJECTION OF BOTULINUM NEUROTOXIN", Toxicon, 34(2), 1996, pp. 237-249

Abstract

The actions of the K+ channel blocker, 3,4-diaminopyridine (3,4-DAP),were studied in the rat extensor digitorum longus (EDL) muscle following local inhibition of neuromuscular transmission by botulinum neurotoxin (BoNT). Local paralysis of the EDL muscle was induced by s.c. injections of BoNT serotypes A, B, E or F over the anterior tibialis muscle, One to 14 days later, the rats were anesthetized with urethane, and isometric twitch tensions following stimulation of the peroneal nerve were measured in situ. Muscles were paralyzed within 24 hr of administration of 5 mouse LD(50) units (U) of BoNT/A and remained inhibited for the entire 14-day period of observation. Similar levels of inhibition, but of shorter duration, were observed after local injection of 20 U of BoNT/E, 10(4) U of BoNT/B or 20 U of BoNT/F. 3,4-DAP (4 mg/kg, i.v.) potentiated twitch tensions markedly in BoNT/A intoxicated muscle. The increase in tension developed rapidly (halftime = 5.81 +/- 0.6 min), persisted for approximately 1 hr, then decayed slowly with a halftime of 25.2 +/- 4.6 min. Subsequent administration of 3,4-DAP restored tensions to the original maxima, and this procedure could be repeated up to eight times with no decrement. The action of 3,4-DAP was comparable when given 1, 2, 3 or 7 days after BoNT/A and enhanced when administered 14 days after toxin injection. 3,4-DAP was less effective inreversing BoNT/E-induced muscle paralysis and nearly ineffective in antagonizing the paralytic actions of BoNT/B or BoNT/F. The results indicate that 3,4-DAP is of benefit in BoNT/A and BoNT/E intoxication, but is of marginal value after exposure to serotypes B and F.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/09/20 alle ore 11:18:10