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Titolo:
CLONING AND EXPRESSION OF A MAMMALIAN GENE INVOLVED IN THE REDUCTION OF METHIONINE SULFOXIDE RESIDUES IN PROTEINS
Autore:
MOSKOVITZ J; WEISSBACH H; BROT N;
Indirizzi:
ROCHE INST MOLEC BIOL,ROCHE RES CTR NUTLEY NJ 07110
Titolo Testata:
Proceedings of the National Academy of Sciences of the United Statesof America
fascicolo: 5, volume: 93, anno: 1996,
pagine: 2095 - 2099
SICI:
0027-8424(1996)93:5<2095:CAEOAM>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
OXIDIZED ALPHA-1-PROTEINASE INHIBITOR; ENZYMATIC REDUCTION; ESCHERICHIA-COLI; BIOCHEMISTRY; PURIFICATION; REDUCTASES; SEQUENCE; FLUID;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
23
Recensione:
Indirizzi per estratti:
Citazione:
J. Moskovitz et al., "CLONING AND EXPRESSION OF A MAMMALIAN GENE INVOLVED IN THE REDUCTION OF METHIONINE SULFOXIDE RESIDUES IN PROTEINS", Proceedings of the National Academy of Sciences of the United Statesof America, 93(5), 1996, pp. 2095-2099

Abstract

An enzyme that reduces methionine sulfoxide [Met(O)] residues In proteins [peptide Met(O) reductase (MsrA), EC 1.8.4.6; originally identified in Escherichia coli] was purified from bovine liver, and the cDNA encoding this enzyme was cloned and sequenced, The mammalian homologue of E. coli msrA (also called pmsR) cDNA encodes a protein of 255 aminoacids with a calculated molecular mass of 25,846 Da, This protein has61% identity with the E. coli MsrA throughout a region encompassing a199-amino acid overlap, The protein has been overexpressed in E. coliand purified to homogeneity. The mammalian recombinant MsrA can use as substrate, proteins containing Met(O) as well as other organic compounds that contain an alkyl sulfoxide group such as N-acetylMet(O), Met(O), and dlmethyl sulfoxide, Northern analysts of rat tissue extracts showed that rat msrA mRNA is present in a variety of organs with the highest level found in kidney. This is consistent with the observation that kidney extracts also contained the highest level of enzyme activity.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/07/20 alle ore 17:35:01