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Titolo:
CELL-CYCLE TRAVERSE IN AHH-1 TK+ - HUMAN LYMPHOBLASTOID-CELLS EXPOSEDTO THE CHROMOSOMAL MUTAGEN, M-AMSA/
Autore:
MORRIS SM; MCGARRITY LJ; DOMON OE; CHEN JJ; CASCIANO DA;
Indirizzi:
US FDA,NATL CTR TOXICOL RES,DIV GENET TOXICOL JEFFERSON AR 72079 US FDA,NATL CTR TOXICOL RES,DIV BIOMETRY & RISK ASSESSMENT JEFFERSON AR 72079
Titolo Testata:
Environmental and molecular mutagenesis
fascicolo: 1, volume: 27, anno: 1996,
pagine: 10 - 18
SICI:
0893-6692(1996)27:1<10:CTIAT->2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
DNA STRAND BREAKS; HAMSTER OVARY CELLS; HL-60 CELLS; S-PHASE; TOPOISOMERASE INHIBITORS; APOPTOTIC CELLS; FLOW-CYTOMETRY; P34CDC2 KINASE; CAMPTOTHECIN; MECHANISM;
Keywords:
CELL CYCLE TRAVERSE; HUMAN LYMPHOBLASTOID CELLS; M-AMSA; FLOW CYTOMETRY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
45
Recensione:
Indirizzi per estratti:
Citazione:
S.M. Morris et al., "CELL-CYCLE TRAVERSE IN AHH-1 TK+ - HUMAN LYMPHOBLASTOID-CELLS EXPOSEDTO THE CHROMOSOMAL MUTAGEN, M-AMSA/", Environmental and molecular mutagenesis, 27(1), 1996, pp. 10-18

Abstract

AHH-1 tk +/- cells were exposed to the chemother apeutic agent, m-amsa, both in complete medium and in medium without serum, subcultured incomplete medium, and the effect on the traverse of the cell cycle determined by flow cytometric analysis of bromodeoxyuridine (BrdUrd)-labeled DNA. After exposure to m-amsa (day 0), the percentage of S-phase cells increased significantly (P < 0.0017) with increasing concentration. Cells also accumulated in G2/M as evidenced by the significant (P <0.0026), concentration-dependent increase in the percentage of cells detected within this phase. Serum deprivation during exposure resultedin significantly (P = 0.024) more cells in S-phase than in cultures exposed to m-amsa in complete medium. After three days in culture, a significant (P = 0.0001) accumulation of cells in G2/M was present; the percentage of cells in G2/M did not differ significantly (P = 0.148) in cultures exposed to m-amsa in complete medium or in serum-free medium. However, a significant (P < 0.001) loss of S-phase cells was found in cultures exposed without serum. At day 7, no significant concentration effects were detected (G0/G1, P = 0.6026; S-phase, P = 0.9773; G2/M, P = 0.8401). These results demonstrate that exposure to m-amsa perturbs the traverse of the cell cycle, initially by inhibiting the completion of S-phase and followed by an accumulation of cells in G2/M. In addition, exposure to m-amsa under conditions of serum deprivation results in an increased percentage of cells in the initial S-phase after exposure, the loss of S-phase cells from the culture after three days,and the appearance of a subdiploid peak, consistent with cells undergoing apoptosis. (C) 1996 Wiley-Liss, Inc.

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Documento generato il 06/04/20 alle ore 23:31:48