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Titolo:
SYNAPTOTAGMIN, A SYNAPTIC VESICLE PROTEIN, IS PRESENT IN HUMAN CEREBROSPINAL-FLUID - A NEW BIOCHEMICAL MARKER FOR SYNAPTIC PATHOLOGY IN ALZHEIMER-DISEASE
Autore:
DAVIDSSON P; JAHN R; BERGQUIST J; EKMAN R; BLENNOW K;
Indirizzi:
GOTHENBURG UNIV,UNIT NEUROCHEM,DEPT CLIN NEUROSCI GOTHENBURG SWEDEN GOTHENBURG UNIV,UNIT NEUROCHEM,DEPT CLIN NEUROSCI GOTHENBURG SWEDEN YALE UNIV,SCH MED,HOWARD HUGHES MED INST,DEPT CELL BIOL NEW HAVEN CT 06510
Titolo Testata:
Molecular and chemical neuropathology
fascicolo: 2, volume: 27, anno: 1996,
pagine: 195 - 210
SICI:
1044-7393(1996)27:2<195:SASVPI>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
DENSE-CORE VESICLES; SENILE DEMENTIA; MEMBRANE-PROTEIN; CEREBRAL-CORTEX; AGE; DISORDERS; CLASSIFICATION; HETEROGENEITY; SYNAPTOPHYSIN; EXOCYTOSIS;
Keywords:
ALZHEIMER DISEASE; CEREBROSPINAL FLUID (CSF); SYNAPSE; SYNAPTOTAGMIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
52
Recensione:
Indirizzi per estratti:
Citazione:
P. Davidsson et al., "SYNAPTOTAGMIN, A SYNAPTIC VESICLE PROTEIN, IS PRESENT IN HUMAN CEREBROSPINAL-FLUID - A NEW BIOCHEMICAL MARKER FOR SYNAPTIC PATHOLOGY IN ALZHEIMER-DISEASE", Molecular and chemical neuropathology, 27(2), 1996, pp. 195-210

Abstract

Using a novel approach, including affinity chromatography, reversed-phase chromatography, and chemiluminescence immuno-blotting, we have for the first time been able to demonstrate one of the small synaptic vesicle proteins, synaptotagmin I, in cerebrospinal fluid (CSF). Two other small synaptic vesicle proteins, rab3a and synaptophysin, were not detectable. The approximate molecular weight of CSF-synaptotagmin was 65 kDa, as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Further characterization of CSF synaptotagminby high-performance capillary electrophoresis (HPCE) showed a single peak. These findings support that the whole synaptotagmin molecule is present in CSF, without significant proteolytic degradation. After high-speed centrifugation of CSF, synaptotagmin was exclusively found in the supernatant, suggesting that synaptotagmin is present in CSF as a free protein, and not as a constituent of synaptic vesicles. In a preliminary study, we found a marked reduction of CSF synaptotagmin in patients with early onset Alzheimer disease (EAD) as compared with age-matched healthy individuals. To elucidate the biological relevance of this finding, we also quantified synaptotagmin in brain tissue. A markedreduction in synaptotagmin was found both in the hippocampus and frontal cortex of EAD, suggesting that a decrease in synaptotagmin in the brain is followed by a concomitant decrease in the CSF. Analysis of CSF synaptotagmin might provide a tool to study synaptic function and pathology in the human brain.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/01/20 alle ore 03:36:42