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Titolo:
NOVEL ANTICOAGULANTS FOR FLOW CYTOMETRIC ANALYSIS OF LIVE LEUKOCYTES IN WHOLE-BLOOD
Autore:
MCCARTHY DA; MACEY MG;
Indirizzi:
UNIV LONDON QUEEN MARY & WESTFIELD COLL,SCH BIOL SCI,MILE END RD LONDON E1 4NS ENGLAND ROYAL MARSDEN HOSP,DEPT HAEMATOL LONDON SW3 6JJ ENGLAND
Titolo Testata:
Cytometry
fascicolo: 3, volume: 23, anno: 1996,
pagine: 196 - 204
SICI:
0196-4763(1996)23:3<196:NAFFCA>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
PLATELET-AGGREGATION; CHLOROMETHYL KETONE; CHEMOTACTIC FACTORS; PLASMA KALLIKREIN; HAGEMAN-FACTOR; FACTOR-XII; NEUTROPHILS; EXPRESSION; LEUKOCYTES; ANTIGENS;
Keywords:
LEUKOCYTE INTEGRINS; ADHESION MOLECULES; APROTININ; AEBSF; PMSF; PPACK; HIRUDIN; ANTICOAGULANTS; FLOW CYTOMETRY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
36
Recensione:
Indirizzi per estratti:
Citazione:
D.A. Mccarthy e M.G. Macey, "NOVEL ANTICOAGULANTS FOR FLOW CYTOMETRIC ANALYSIS OF LIVE LEUKOCYTES IN WHOLE-BLOOD", Cytometry, 23(3), 1996, pp. 196-204

Abstract

Enzyme inhibitors have been compared with conventional anticoagulants; for the flow cytometric analysis of Live leucocytes in whole blood. At 18 to 20 degrees C in vitro, PPACK (60 mu M), hirudin (10 units ml(-1)), leupeptin (20 mu g ml(-1)) and aprotinin (50 mu g ml(-1)) inhibited blood clotting for 4 h or more, whereas PMSF (8 mg ml(-1)) or AEBSF (5 mg ml(-1)) inhibited clotting for only 20 or 40 min, respectively, When labelled with CD11b antibodies and analysed immediately ex vivoat 4 degrees C, the percentages of lymphocytes, monocytes, and polymorphs which stained positively and their mean fluorescence intensities were similar, irrespective into which anticoagulant blood was collected, Less than 1.5-fold increases in expression occurred on monocytes and polymorphs when blood anticoagulated with enzyme inhibitors or conventional anticoagulants was kept at 4 degrees C, or when blood anticoagulated with citrate, heparin, or hirudin was kept at 18 to 20 degrees C for 1 h before labelling and analysis, whereas approximately 2-fold increases in expression occurred in blood kept with K(3)EDTA, leupeptin, or aprotinin and more than 3-fold increases in blood kept with AEBSF or PPACK at 18 to 20 degrees C for 1 h, Further studies showed that leupeptin could be used effectively as the anticoagulant when investigating functional responses of live leucocytes in whole blood samples by now cytometry and for the isolation of leucocytes with minimal modulation of adhesion molecules. (C) 1996 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/01/21 alle ore 10:06:10