Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
ACTIVATION OF THE CAMP-DEPENDENT SIGNALING PATHWAY DOWN-REGULATES THEEXPRESSION OF INTERLEUKIN-3 AND GRANULOCYTE-MACROPHAGE COLONY-STIMULATING FACTOR IN ACTIVATED HUMAN T-LYMPHOCYTES
Autore:
BORGER P; KAUFFMAN HF; VIJGEN JLJ; POSTMA DS; VELLENGA E;
Indirizzi:
UNIV GRONINGEN HOSP,DEPT HEMATOL,OOSTERSINGEL 59 9713 EZ GRONINGEN NETHERLANDS UNIV GRONINGEN,DEPT MED,DIV ALLERGOL GRONINGEN NETHERLANDS UNIV GRONINGEN,DEPT MED,DIV PULMONOL GRONINGEN NETHERLANDS UNIV GRONINGEN,DEPT MED,DIV HEMATOL GRONINGEN NETHERLANDS
Titolo Testata:
Experimental hematology
fascicolo: 2, volume: 24, anno: 1996,
pagine: 108 - 115
SICI:
0301-472X(1996)24:2<108:AOTCSP>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
CSF MESSENGER-RNA; ADENYLATE-CYCLASE; GENE-EXPRESSION; POSTTRANSCRIPTIONAL REGULATION; 3'-UNTRANSLATED REGION; ASTHMATIC-PATIENTS; BINDING-PROTEIN; CELLS; PHOSPHORYLATION; IDENTIFICATION;
Keywords:
T LYMPHOCYTES; IL-3; GM-CSF; CAMP-DEPENDENT SIGNALING PATHWAY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
33
Recensione:
Indirizzi per estratti:
Citazione:
P. Borger et al., "ACTIVATION OF THE CAMP-DEPENDENT SIGNALING PATHWAY DOWN-REGULATES THEEXPRESSION OF INTERLEUKIN-3 AND GRANULOCYTE-MACROPHAGE COLONY-STIMULATING FACTOR IN ACTIVATED HUMAN T-LYMPHOCYTES", Experimental hematology, 24(2), 1996, pp. 108-115

Abstract

Expression of cytokines by T lymphocytes is a highly balanced process, involving stimulatory and inhibitory intracellular signaling pathways. We have examined the modulating effects of the cAMP-dependent signaling pathway on the expression of interleukin-3 (IL-3) and granulocyte-macrophage colony-stimulating factor (GM-CSF) in activated human T lymphocytes. 2'-O-dibutyryl-cAMP (db-cAMP), prostaglandin E(2) (PGE(2)),isoproterenol (ISO), and isobutyl methyl-xantin (IBMX) costimulated with concanavalin A (Con A) or Con A plus the phorbolester phorbol myristate acetate (PMA) inhibited IL-3 and GM-CSF mRNA accumulation compared to the effects of Con A or Con A plus PMA alone. Nuclear run-on experiments revealed that the inhibitory effect of db-cAMP could partially be ascribed to a five-fold reduction in transcription rate of both the IL-3 and GM-CSF gene in the presence of Con A or Con A plus PMA. mRNA stability studies demonstrated that PMA increased the stability of both transcripts. db-cAMP did not affect the stability of IL-3 and GM-CSF mRNAs in Con A activated cells. In contrast, in Con A plus PMA activated cells, db-cAMP significantly reduced the half-life of both transcripts: IL-3 >240 minutes vs. 90 minutes and GM-CSF 90 minutes vs. 60minutes. Finally, in accordance with the mRNA data, db-cAMP, PGE(2), and ISO reduced the secretion of IL-3 and GM-CSF protein in Con A and Con A plus PMA activated cells. In conclusion, these data demonstrate that the protein kinase A (PKA)-dependent signaling pathway is an important regulatory mechanism in controlling IL-3 and GM-CSF gene expression in activated human T lymphocytes.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/07/20 alle ore 12:01:30