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Titolo:
NORMAL HUMAN PERIPHERAL-BLOOD MONONUCLEAR-CELLS MOBILIZED WITH GRANULOCYTE-COLONY-STIMULATING FACTOR HAVE INCREASED OSTEOCLASTOGENIC POTENTIAL COMPARED TO NONMOBILIZED BLOOD
Autore:
PURTON LE; LEE MY; TOROKSTORB B;
Indirizzi:
FRED HUTCHINSON CANC RES CTR,DIV CLIN RES,PROGRAM TRANSPLANTAT BIOL,1124 COLUMBIA ST,M-318 SEATTLE WA 98104 UNIV WASHINGTON,DEPT BIOL STRUCT SEATTLE WA 00000 UNIV WASHINGTON,DEPT MED SEATTLE WA 00000
Titolo Testata:
Blood
fascicolo: 5, volume: 87, anno: 1996,
pagine: 1802 - 1808
SICI:
0006-4971(1996)87:5<1802:NHPMMW>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
BONE-MARROW; MULTINUCLEATED CELLS; IDENTIFICATION; DIFFERENTIATION; GENERATION; PRECURSORS; CULTURES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
25
Recensione:
Indirizzi per estratti:
Citazione:
L.E. Purton et al., "NORMAL HUMAN PERIPHERAL-BLOOD MONONUCLEAR-CELLS MOBILIZED WITH GRANULOCYTE-COLONY-STIMULATING FACTOR HAVE INCREASED OSTEOCLASTOGENIC POTENTIAL COMPARED TO NONMOBILIZED BLOOD", Blood, 87(5), 1996, pp. 1802-1808

Abstract

Single-cell suspensions of granulocyte colony-stimulating factor (G-CSF)-mobilized peripheral blood mononuclear cells (G-PBMC) cultured in alpha minimal essential medium (alpha MEM) containing 10% fetal bovineserum formed multicellular aggregates within 24 hours. In six separate experiments, formation of aggregates appeared to be dependent on cell density per surface area, so that 5.8 +/- 1.3 aggregates formed per 1 x 10(5) cells when G-PBMC were cultured at densities greater than orequal to 1 x 10(5) cells/cm(2). The frequency of aggregate formation was less than 1 per 10(5) cells when G-PBMC were cultured at densitiesless than 1 x 10(5) cells/cm(2). Once formed, aggregates became adherent within 72 hours, and then, over the course of 21 days, released CD3/CD4/CD25-positive cells into the supernatant. This T-cell productionpeaked between days 7 and 14, reaching a total of 1,269 +/- 125.9 cells released per aggregate by day 21. Between days 14 and 21, the aggregates also generated macroscopic clusters of adherent mononuclear and giant multinucleated cells that stained positive for tartrate-resistant acid phosphatase (TRAP). At 4 weeks, the macroscopic foci coalesced into monolayers. Multinucleated TRAP-positive cells were distinguishedfrom macrophage polykaryons by the absence of CD14 expression and thepresence of osteoclast-specific membrane receptors for calcitonin andalpha(v) beta(3)-vitronectin. The osteoclast nature of these cells was further demonstrated by their ability to form resorption lacunae on dentine slices. Comparable osteoclast formation was not detected in cultures of normal marrow or normal nonmobilized peripheral blood. (C) 1996 by The American Society of Hematology.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/04/20 alle ore 09:15:08