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Titolo:
EFFECTS OF CELL-CYCLE-ARREST AGENTS ON CLEAVAGE AND DEVELOPMENT OF MOUSE EMBRYOS
Autore:
SAMAKE S; SMITH LC;
Indirizzi:
UNIV MONTREAL,FAC VET MED,CTR RECH REPROD ANIM ST HYACINTHE PQ J2S 7C6 CANADA UNIV MONTREAL,FAC VET MED,CTR RECH REPROD ANIM ST HYACINTHE PQ J2S 7C6 CANADA
Titolo Testata:
The Journal of experimental zoology
fascicolo: 2, volume: 274, anno: 1996,
pagine: 111 - 120
SICI:
0022-104X(1996)274:2<111:EOCAOC>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
NUCLEAR TRANSPLANTATION; DNA-REPLICATION; PROTEIN-PHOSPHORYLATION; ENUCLEATED OOCYTES; MEIOTIC MATURATION; 6-DIMETHYLAMINOPURINE; DIVISION; STAGE; STARFISH; INVITRO;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
40
Recensione:
Indirizzi per estratti:
Citazione:
S. Samake e L.C. Smith, "EFFECTS OF CELL-CYCLE-ARREST AGENTS ON CLEAVAGE AND DEVELOPMENT OF MOUSE EMBRYOS", The Journal of experimental zoology, 274(2), 1996, pp. 111-120

Abstract

In mammals, there are no reliable methods for synchronizing cell division of early embryos without reducing their ability to develop into blastocysts and fetuses. The present study was undertaken to examine the in vitro inhibition of cell division of four-cell mouse embryos by cell cycle arrest agents. The reversibility of the agents was also tested by examining the developmental ability of treated embryos. Four-cell mouse embryos obtained at 54 hr post-human chorionic gonadotrophin (post-hCG) were cultured for 4, 8, 12, or 16 hr in media supplemented with either nocodazole, an inhibitor of tubulin polymerization, 6-dimethylaminopurine (6-DMAP), an inhibitor of maturation promoting factor (MPF) activation, or aphidicolin, a specific inhibitor of DNA polymerase alpha. Reversibility and toxicity of these agents were both dose andtime dependent. For all three agents, prolonging cleavage arrest for 8 or 16 hr (at the effective concentrations) caused embryo lethality. Although nocodazole treatment was least cytotoxic, 6-DMAP and aphidicolin concentrations which induce cleavage arrest were detrimental to development beyond the blastocyst stage. The results of this study show that the development of embryos treated with these three cell-cycle-arrest agents is dose and incubation time dependent. Toxic effects beyond the blastocyst stage could only be minimized for nocodazole by reducing the exposure time of treatment and concentration of the mitotic inhibitor. However, these results render doubt on the usefulness of 6-DMAP and aphidicolin for synchronization studies leading to embryo transfer procedures. (C) 1996 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/12/20 alle ore 04:35:11