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Titolo:
RESTRICTION-FRAGMENT-LENGTH-POLYMORPHISM OF GENES OF THE ALPHA-2(XI) COLLAGEN, BONE MORPHOGENETIC PROTEIN-2, ALKALINE-PHOSPHATASE, AND TUMOR-NECROSIS-FACTOR-ALPHA AMONG PATIENTS WITH OSSIFICATION OF POSTERIOR LONGITUDINAL LIGAMENT AND CONTROLS FROM THE JAPANESE POPULATION
Autore:
KOGA H; HAYASHI K; TAKETOMI E; MATSUNAGA S; YASHIKI S; FUJIYOSHI T; SONODA S; SAKOU T;
Indirizzi:
KAGOSHIMA UNIV,FAC MED,DEPT ORTHOPED SURG,8-35-1 SAKURAGAOKA KAGOSHIMA 890 JAPAN KAGOSHIMA UNIV,FAC MED,DEPT ORTHOPED SURG KAGOSHIMA 890 JAPAN KAGOSHIMA UNIV,FAC MED,DEPT VIROL KAGOSHIMA 890 JAPAN
Titolo Testata:
Spine (Philadelphia, Pa. 1976)
fascicolo: 4, volume: 21, anno: 1996,
pagine: 469 - 473
SICI:
0362-2436(1996)21:4<469:ROGOTA>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
CERVICAL-SPINE; COL-11A2 GENE; CELLS; RFLP; DNA; EXPRESSION;
Keywords:
ALKALINE PHOSPHATASE; BONE MORPHOGENETIC PROTEIN-2; ALPHA-2(XI) COLLAGEN; OSSIFICATION OF THE POSTERIOR LONGITUDINAL LIGAMENT; RESTRICTION FRAGMENT LENGTH POLYMORPHISM; TUMOR NECROSIS FACTOR-ALPHA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
41
Recensione:
Indirizzi per estratti:
Citazione:
H. Koga et al., "RESTRICTION-FRAGMENT-LENGTH-POLYMORPHISM OF GENES OF THE ALPHA-2(XI) COLLAGEN, BONE MORPHOGENETIC PROTEIN-2, ALKALINE-PHOSPHATASE, AND TUMOR-NECROSIS-FACTOR-ALPHA AMONG PATIENTS WITH OSSIFICATION OF POSTERIOR LONGITUDINAL LIGAMENT AND CONTROLS FROM THE JAPANESE POPULATION", Spine (Philadelphia, Pa. 1976), 21(4), 1996, pp. 469-473

Abstract

Study Design. The present study analyzed the restriction fragment length polymorphism patterns of alpha 2(XI) collagen, bone morphogenetic protein-2, alkaline phosphatase, and tumor necrosis factor-alpha genesin patients with ossification of the posterior longitudinal ligament. This study investigates the genetic polymorphism of bone-induced factors in patients with ossification of the posterior longitudinal ligament and compares it with healthy control subjects.Objectives. To clarify the genetic markers linked to ossification of the posterior longitudinal ligament. Summary of Background Data. Ossification of the posterior longitudinal ligament is a genetic disease associated with abnormalcalcium metabolism involving the posterior longitudinal ligament. Previous genetic studies have not identified the pathologic mechanism of ossification of the posterior longitudinal ligament. Histopathologic studies of ossification of the posterior longitudinal ligament and the animal model, the spinal hyperostotic mouse, have revealed an increasein Type XI collagen and bone morphogenetic protein-2 expression. Methods. Eighteen Japanese patients with ossification of the posterior longitudinal ligament and 51 healthy, unrelated control subjects were investigated for the restriction fragment length polymorphism patterns ofCOL11A2, bone morphogenetic protein-2, alkaline phosphatase, and tumor necrosis factor-alpha, genes with various restriction endonucleases. Results. The gene frequencies of COL11A2 obtained with BamHI (10.0 kbfragment) and HindIII (19.0 kb fragment) observed in patients with ossification of the posterior longitudinal ligament were higher comparedwith control subjects (0.43 and 0.14, respectively). These differences were statistically significant (BamHI P = 0.018; HindIII P = 0.046). Two new restriction fragment length polymorphism patterns were detected of the bone morphogenetic protein-2 gene with MspI and TaqI and onealready known restriction fragment length polymorphism pattern of thetumor necrosis factor-alpha gene with Ncol. However, they were not significantly different from the control subjects. Conclusion. Seven restriction fragment length polymorphisms of COL11A2 gene were identified. Two of them (BamHI, 10.0/10.0 kb genotype; HindIII, 19.0/19.0 kb genotype) were significantly different in patients with ossification of the posterior longitudinal ligament.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/09/20 alle ore 18:39:03