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Titolo:
PURIFICATION AND SOME PROPERTIES OF A BETA-XYLOSIDASE AND AN ALPHA-FUCOSIDASE FROM APPLE SNAILS (POMACEA-CANALICULATA)
Autore:
HIRATA K; NAKAHARA Y; KIMURA Y; FUNATSU G;
Indirizzi:
KYUSHU UNIV,FAC AGR,LAB PROT CHEM & ENGN FUKUOKA 812 JAPAN KYUSHU UNIV,FAC AGR,LAB PROT CHEM & ENGN FUKUOKA 812 JAPAN OKAYAMA UNIV,FAC AGR,DEPT BIORESOURCES CHEM OKAYAMA 700 JAPAN
Titolo Testata:
Bioscience, biotechnology, and biochemistry
fascicolo: 2, volume: 60, anno: 1996,
pagine: 249 - 254
SICI:
0916-8451(1996)60:2<249:PASPOA>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
OLIGOSACCHARIDES; PROTEINS;
Keywords:
BETA-XYLOSIDASE; ALPHA-FUCOSIDASE; APPLE SNAIL; OLIGOSACCHARIDE STRUCTURE; EXOGLYCOSIDASE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
13
Recensione:
Indirizzi per estratti:
Citazione:
K. Hirata et al., "PURIFICATION AND SOME PROPERTIES OF A BETA-XYLOSIDASE AND AN ALPHA-FUCOSIDASE FROM APPLE SNAILS (POMACEA-CANALICULATA)", Bioscience, biotechnology, and biochemistry, 60(2), 1996, pp. 249-254

Abstract

beta-Xylosidase and alpha-fucosidase were purified from the viscera of apple snails (Pomacea canaliculata) using ammonium sulfate precipitation, hydrophobic chromatography on Butyl-Toyopearl, gel filtration onSephacryl S-300, and Q-Sepharose column chromatography, beta-Xylosidase and alpha-fucosidase are glycoproteins and their molecular masses were estimated to be approximately 85 kDa and 54 kDa by SDS-polyacrylamide gel electrophoresis, and assumed to be about 96 kDa and 230 kDa from their sedimentation coefficients, respectively, indicating that theformer is a monomer and the latter has a tetrameric structure, beta-Xylosidase is stable at pH 4-10 and its optimum pH toward pNP-beta-D-xyloside is around 3.6 while alpha-fucosidase is fairly stable at 65 degrees C and pH 4-10, and its optimum pHs toward pNP-alpha-L-fucoside are around 3.2 and 5.2, beta-Xylosidase released a xylose residue from Xyl beta 1 --> 2Man beta 1 --> 4GlcNAc beta 1 --> 4(Fuc alpha 1 --> 3)GlcNAc-PA and Man alpha 1 --> 6(Man alpha 1 --> 3)(Xyl beta 1 --> 2)Manbeta 1 --> 4GlcNAc beta 1 --> 4(Fuc alpha 1 --> 3)GlcNAc-PA, but not from GlcNAc beta 1 --> 2Man alpha 1 --> 6(GlcNAc beta 1 --> 2Man alpha1 --> 3)(Xyl beta 1 --> 2)Man beta 1 --> 4GlcNAc beta 1 --> 4(Fuc alpha 1 --> 3)GlcNAc-PA, while alpha-fucosidase released a fucose residuefrom these three PA-oligosaccharides.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/09/20 alle ore 01:03:22