Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
MOLECULAR-PROPERTIES OF CHIMERICAL MUTANTS OF GECKO BLUE AND BOVINE RHODOPSIN
Autore:
KOJIMA D; OURA T; HISATOMI O; TOKUNAGA F; FUKADA Y; YOSHIZAWA T; SHICHIDA Y;
Indirizzi:
KYOTO UNIV,FAC SCI,DEPT BIOPHYS,SAKYO KU KYOTO 60601 JAPAN KYOTO UNIV,FAC SCI,DEPT BIOPHYS,SAKYO KU KYOTO 60601 JAPAN OSAKA UNIV,FAC SCI,DEPT EARTH & SPACE SCI TOYONAKA OSAKA 560 JAPAN UNIV TOKYO,GRAD SCH ARTS & SCI,DEPT LIFE SCI TOKYO 153 JAPAN OSAKA SANGYO UNIV,FAC ENGN,DEPT INFORMAT SYST ENGN DAITO OSAKA 574 JAPAN
Titolo Testata:
Biochemistry
fascicolo: 8, volume: 35, anno: 1996,
pagine: 2625 - 2629
SICI:
0006-2960(1996)35:8<2625:MOCMOG>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
CONE VISUAL PIGMENTS; AMINO-ACIDS; METARHODOPSIN-II; RED; SPECTROSCOPY; PROTONATION; ACTIVATION; PHOTOLYSIS; SEGMENT; SPECTRA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
33
Recensione:
Indirizzi per estratti:
Citazione:
D. Kojima et al., "MOLECULAR-PROPERTIES OF CHIMERICAL MUTANTS OF GECKO BLUE AND BOVINE RHODOPSIN", Biochemistry, 35(8), 1996, pp. 2625-2629

Abstract

In spite of the high similarity in amino acid sequence between rod visual pigment rhodopsin and gecko blue-sensitive pigment (gecko blue), not only the spectral sensitivities but also the thermal decay rates of the meta II- and III-intermediates are noticeably different from oneanother [Kojima et al. (1995) Biochemistry 34, 1096-1106]. In order to identify the protein region(s) that contain(s) key residues being responsible for the functional difference, we constructed six chimericalmutants derived from gecko blue and bovine rhodopsin, with the aid ofprotein production in a human embryonic kidney cell line (2933). While the absorption maximum of every mutant was located in between gecko blue (466 nm) and bovine rhodopsin (500 nm), a large blue-shift (18 nm) was observed when the helices I-III of rhodopsin were replaced with those of gecko blue. A lime-resolved spectroscopic study demonstrated that this replacement also accelerated the decay rate of the meta II-intermediate. The decay of the meta III-intermediate of the mutants became faster as the compartment of gecko blue was increased. Thus, the faster decay of the meta II-intermediate of gecko blue is largely attributed to residues within helices I-III, while the decay of the meta III-intermediate apparently depends on the overall structure of the protein.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 21:04:05