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Titolo:
FACTORS AFFECTING YIELDS OF ALPHA-HORMONES OF PHYTOPHTHORA-PARASITICAOBTAINED BY ADSORPTION
Autore:
CHERN LL; KO WH; TANG CS;
Indirizzi:
UNIV HAWAII MANOA,BEAUMONT AGR RES CTR,DEPT PLANT PATHOL HILO HI 96720 UNIV HAWAII MANOA,BEAUMONT AGR RES CTR,DEPT PLANT PATHOL HILO HI 96720 UNIV HAWAII MANOA,DEPT ENVIRONM BIOCHEM HONOLULU HI 96822
Titolo Testata:
Canadian journal of microbiology
fascicolo: 2, volume: 42, anno: 1996,
pagine: 172 - 176
SICI:
0008-4166(1996)42:2<172:FAYOAO>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
SEXUAL REPRODUCTION; INFESTANS;
Keywords:
SEX HORMONE; HORMONE PRODUCTION; SEXUAL REPRODUCTION; PHYTOPHTHORA PARASITICA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
12
Recensione:
Indirizzi per estratti:
Citazione:
L.L. Chern et al., "FACTORS AFFECTING YIELDS OF ALPHA-HORMONES OF PHYTOPHTHORA-PARASITICAOBTAINED BY ADSORPTION", Canadian journal of microbiology, 42(2), 1996, pp. 172-176

Abstract

The conditions for improved yields of the alpha hormones of Phytophthora parasitica compared with those of the original technique established by Ko are described. Both hormones alpha 1 and alpha 2 were adsorbed on the Millipore filter by exposing both sides of the filter directly to freshly inoculated 40% V-8 agar blocks for 4 days. Usage of polycarbonate membrane in the original method to maintain sterility of the filter was replaced by sterilization of the filter with ethanol. Amongfive organic solvents tested, 95% ethanol was the most effective in extracting both alpha hormones from the Millipore filter. A 1-min shaking of the hormone-loaded filter with 95% ethanol was sufficient to extract ca. 95% of hormones alpha 1 and alpha 2. Results on the solubility properties suggest that the alpha hormones of Phytophthora parasitica are lipid-like natural products and that hormone alpha 2 is more polar than hormone alpha 1.

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Documento generato il 08/07/20 alle ore 00:47:05