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Titolo:
COMPARATIVE EFFECTS OF CD2-METALLOTHIONEIN ON CULTURED KIDNEY TUBULE CELLS( AND CD)
Autore:
BLUMENTHAL S; LEWAND D; KREZOSKI SK; PETERING DH;
Indirizzi:
WOOD VET HOSP,DEPT NEPHROL MILWAUKEE WI 53226 MED COLL WISCONSIN,DEPT NEPHROL MILWAUKEE WI 53226 UNIV WISCONSIN,DEPT CHEM MILWAUKEE WI 53201
Titolo Testata:
Toxicology and applied pharmacology
fascicolo: 2, volume: 136, anno: 1996,
pagine: 220 - 228
SICI:
0041-008X(1996)136:2<220:CEOCOC>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
CADMIUM-METALLOTHIONEIN; LLC-PK1 CELLS; PHLORIZIN-BINDING; EXPOSED RATS; MOUSE KIDNEY; PROTEIN; NEPHROTOXICITY; HEPATOTOXICITY; ACCUMULATION; TRANSPORT;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
38
Recensione:
Indirizzi per estratti:
Citazione:
S. Blumenthal et al., "COMPARATIVE EFFECTS OF CD2-METALLOTHIONEIN ON CULTURED KIDNEY TUBULE CELLS( AND CD)", Toxicology and applied pharmacology, 136(2), 1996, pp. 220-228

Abstract

The effects of Cd2+ and Cd-metallothionein on two cultured cells withproximal tubule characteristics, mouse kidney cortical cells and pig kidney LLC-PK1 cells, have been compared. Cd2+ inhibits Na+-glucose cotransport in LLC-PK1 cells and in the process decreases the number of binding sites for [H-3]phlorizin, a competitive inhibitor of glucose for the Na+-glucose cotransporter, During 24 hr incubation and over a range of concentrations in the two cell types, only Cd2+ inhibited Na+-glucose cotransport even when approximately equal concentrations of intracellular Cd resulted from these treatments, Indeed, at low concentrations of Cd-metallothionein in mouse cells, transporter activity was elevated. Extension of incubations to 72 hr in mouse cells led to increased Cd uptake and reduction in cell density with both sources of Cd but only a progressive decline in Na+-glucose cotransport activity with Cd2+. Zn-metallothionein was without effect under comparable conditions. Both forms of Cd were accumulated by these cells, with the large majority of the metal ion localizing in metallothionein as a Cd, Zn-protein in LLC-PK1 cells. Under equal exposure conditions, the net uptake of Cd from Cd2+ was an order of magnitude greater than Cd from Cd-metallothionein in the two cell types. It is evident that the mechanismsof toxicity of Cd2+ and Cd-metallothionein as well as their modes of uptake differ in these two cell types. (C) 1996 Academic Press, Inc.

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Documento generato il 30/11/20 alle ore 07:04:26