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Titolo:
QUANTITATION OF THE NUCLEOPHOSMIN B23-TRANSLOCATION USING IMAGING ANALYSIS/
Autore:
CHAN PK; QI Y; AMLEY J; KOLLER CA;
Indirizzi:
BAYLOR COLL MED,DEPT PHARMACOL,1 BAYLOR PLAZA HOUSTON TX 77030 MD ANDERSON HOSP & TUMOR INST,DEPT HEMATOL HOUSTON TX 77030
Titolo Testata:
Cancer letters
fascicolo: 1-2, volume: 100, anno: 1996,
pagine: 191 - 197
SICI:
0304-3835(1996)100:1-2<191:QOTNBU>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
NUCLEOLAR PROTEIN-B23 TRANSLOCATION; MURINE TUMOR-CELLS; HELA-CELLS; RNA-SYNTHESIS; B23-TRANSLOCATION; B23; PHOSPHOPROTEIN-B23; LOCALIZATION; GROWTH; PI5.1;
Keywords:
NUCLEOPHOSMIN; NUCLEOPHOSMIN/B23-TRANSLOCATION; ANTHRACYCLINES; ACTINOMYCIN D; IMMUNOFLUORESCENCE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
14
Recensione:
Indirizzi per estratti:
Citazione:
P.K. Chan et al., "QUANTITATION OF THE NUCLEOPHOSMIN B23-TRANSLOCATION USING IMAGING ANALYSIS/", Cancer letters, 100(1-2), 1996, pp. 191-197

Abstract

We have previously detected by immunofluorescent assay that the cellular localization of nucleophosmin/B23 (NPM) shifts from the nucleolus to the nucleoplasm (NPM-translocation) after exposure of cells to multiple agents. In order to improve the quantification of the NPM-translocation, we have developed a digital imaging technique. Human Lo leukemia cells, MCF-7 breast carcinoma cells, and fresh human leukemia cellswere exposed to anthracyclines or actinomycin D for 4 h. The degree of NPM-translocation was determined and presented as the localization index (LI). Control cells had a LI of about 10, which indicates that the majority of NPM was localized in nucleoli. The LI for drug-treated cells decreased in a dosage- and time-dependent manner. The effect of two classes of anthracycline (daunomycin and aclacinomycin A) and different types of intercalators (daunomycin and actinomycin D) had additive effects on induction of NPM-translocation. The imaging procedure waseasily applied to fresh leukemia cells, thus providing useful information regarding drug effects on cancer cells.

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Documento generato il 23/09/20 alle ore 20:57:27