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Titolo:
INTERLEUKIN-12 DECREASES HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 REPLICATION IN HUMAN MACROPHAGE CULTURES RECONSTITUTED WITH AUTOLOGOUS PERIPHERAL-BLOOD MONONUCLEAR-CELLS
Autore:
AKRIDGE RE; REED SG;
Indirizzi:
INFECT DIS RES INST,1124 COLUMBIA ST,SUITE 464 SEATTLE WA 98104 INFECT DIS RES INST SEATTLE WA 98104
Titolo Testata:
The Journal of infectious diseases
fascicolo: 3, volume: 173, anno: 1996,
pagine: 559 - 564
SICI:
0022-1899(1996)173:3<559:IDHTR>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
STIMULATORY FACTOR NKSF; HIV-INFECTION; INTERFERON-GAMMA; LEISHMANIA-MAJOR; MONOCYTES; PHAGOCYTES; CYTOKINES; IDENTIFICATION; PROLIFERATION; PURIFICATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
30
Recensione:
Indirizzi per estratti:
Citazione:
R.E. Akridge e S.G. Reed, "INTERLEUKIN-12 DECREASES HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 REPLICATION IN HUMAN MACROPHAGE CULTURES RECONSTITUTED WITH AUTOLOGOUS PERIPHERAL-BLOOD MONONUCLEAR-CELLS", The Journal of infectious diseases, 173(3), 1996, pp. 559-564

Abstract

In vitro interactions between interleukin (IL)-12, interferon (IFN)-gamma, and human immunodeficiency virus (HIV) type 1 infection in humanmacrophages were examined. Macrophages were infected with HIV-1 and cocultured with autologous monocyte-depleted peripheral blood mononuclear cells (PBMC). The addition of autologous PBMC to HIV-l-infected macrophages resulted in an expansive increase in reverse transcriptase (RT) activity; however, when both autologous PBMC and IL-12 were added, RT activity decreased (75%-90%) and high levels of IFN-gamma (9-16 ng/mL) were detected. The addition of anti-IFN-gamma antibodies blocked the IL-12-induced decrease in RT activity. Surprisingly, exogenous IL-12 added to HIV-infected macrophage cultures without autologous lymphocytes resulted in a 50%-60% reduction in RT activity and no detectable increase in IFN-gamma. The addition of anti-IFN-gamma did not inhibit this IL-12-mediated effect. These results suggest that IL-12 is capable of indirectly downregulating HIV proliferation in macrophage cultures reconstituted with autologous PBMC and of directly suppressing HIV replication in purified macrophage cultures.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 22:22:10