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Titolo:
TRANSPLANTATION OF HUMAN FETAL TISSUE FROM SPONTANEOUS-ABORTIONS TO ARODENT MODEL OF PARKINSONS-DISEASE
Autore:
KONDOH T; PUNDT LL; BLOUNT JP; CONRAD JA; LOW WC;
Indirizzi:
UNIV MINNESOTA,SCH MED,DEPT NEUROSURG,LIONS RES BLDG,RM 421,2001 6TH ST SE MINNEAPOLIS MN 55455 UNIV MINNESOTA,SCH MED,DEPT NEUROSURG MINNEAPOLIS MN 55455 UNIV MINNESOTA,SCH MED,DEPT PHYSIOL MINNEAPOLIS MN 55455 UNIV MINNESOTA,SCH MED,PROGRAM NEUROSCI MINNEAPOLIS MN 55455
Titolo Testata:
Cell transplantation
fascicolo: 1, volume: 5, anno: 1996,
pagine: 69 - 75
SICI:
0963-6897(1996)5:1<69:TOHFTF>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
NIGROSTRIATAL DOPAMINE DEFICIENCY; MESENCEPHALIC TISSUE; VENTRAL MESENCEPHALON; DENERVATED STRIATUM; CAUDATE-NUCLEUS; ATHYMIC RATS; FOLLOW-UP; NEURONS; GRAFTS; CELLS;
Keywords:
HUMAN FETUS; PARKINSONS DISEASE; SPONTANEOUS ABORTION; TISSUE BANK; TRANSPLANTATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
53
Recensione:
Indirizzi per estratti:
Citazione:
T. Kondoh et al., "TRANSPLANTATION OF HUMAN FETAL TISSUE FROM SPONTANEOUS-ABORTIONS TO ARODENT MODEL OF PARKINSONS-DISEASE", Cell transplantation, 5(1), 1996, pp. 69-75

Abstract

The use of human fetal tissue from elective abortions for cell transplantation therapies has been the subject of considerable controversy. Because of concerns regarding the use of tissue from elective abortions, tissue from spontaneous abortions has been suggested as an alternate donor source. In the present study we have evaluated human fetal tissue from spontaneous abortions to assess its viability, growth potential, and functional expression. Viable cells (Grade I) from a donor (7 wk postconception) were transplanted as a suspension into the striatumof rats with unilateral 6-OHDA lesions of the nigrostriatal pathway. A second group of animals received solid grafts of tissue from a GradeI donor 14 wk postconception. Tissue from Grade II and III specimens were not sufficiently viable for transplantation. Locomotor responses were monitored over a period of 15 wk and revealed an amelioration of rotational asymmetry by animals that received tissue from the 7 wk donor, Animals receiving tissue from the 14 wk donor showed no functionalimprovement. We found numerous graft-derived tyrosine hydroxylase (TH) immunopositive neurons contained within the transplantation site, and a rich plexus of TH-immunopositive fibers extending into the striatum of animals receiving tissue from the 7 wk donor. Animals receiving tissue from the 14 wk donor exhibited tissue necrosis at the transplantsite and mere devoid of TH-immunopositive neurons. These results suggest that human fetal ventral mesencephalic cells from spontaneous abortions can survive and develop after transplantation, and rectify locomotor deficits associated with experimental parkinsonism if the donor tissue is of the appropriate gestational age at the time of implantation. Our study further suggests, however, that the availability of tissue from spontaneous abortions of sufficient viability is quite limited and may thus restrict its potential use in cell transplantation therapies for Parkinson's disease.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/12/20 alle ore 13:01:51