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Titolo:
A NOVEL SITE IN THE MUSCLE CREATINE-KINASE ENHANCER IS REQUIRED FOR EXPRESSION IN SKELETAL BUT NOT CARDIAC-MUSCLE
Autore:
FABRESUVER C; HAUSCHKA SD;
Indirizzi:
UNIV WASHINGTON,DEPT BIOCHEM,357350 SEATTLE WA 98195 UNIV WASHINGTON,DEPT BIOCHEM SEATTLE WA 98195
Titolo Testata:
The Journal of biological chemistry
fascicolo: 9, volume: 271, anno: 1996,
pagine: 4646 - 4652
SICI:
0021-9258(1996)271:9<4646:ANSITM>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
MYOSIN HEAVY-CHAIN; HELIX-LOOP-HELIX; CAT BINDING-FACTOR; GENE-EXPRESSION; DNA-BINDING; REGULATORY ELEMENTS; NUCLEAR FACTOR; MYC HOMOLOGY; BETA GENE; FACTOR-II;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
64
Recensione:
Indirizzi per estratti:
Citazione:
C. Fabresuver e S.D. Hauschka, "A NOVEL SITE IN THE MUSCLE CREATINE-KINASE ENHANCER IS REQUIRED FOR EXPRESSION IN SKELETAL BUT NOT CARDIAC-MUSCLE", The Journal of biological chemistry, 271(9), 1996, pp. 4646-4652

Abstract

Expression of the muscle creatine kinase (MCK) gene in skeletal and heart muscle is controlled in part by a 5' tissue-specific enhancer. Inorder to identify new regulatory elements, we designed mutations in apreviously untested conserved portion of this enhancer. Transfection analysis of these mutations delineated a new control element, named Trex (Transcriptional regulatory element x), which is required for full transcriptional activity of the MCK enhancer in skeletal but not cardiac muscle cells. Gel mobility shift assays demonstrate that myocyte, myoblast, and fibroblast nuclear extracts but not primary cardiomyocytenuclear extracts contain a trans-acting factor that binds specifically to Trex. The Trex sequence is similar (7/8 bases) to the TEF-1 consensus DNA binding site involved in regulating other muscle genes. To determine if TEF-1 interacts with Trex, selected TEF-1 binding sites such as GTIIc and M-CAT and two anti-TEF-1 antisera were used in gel shift assays. These experiments strongly suggest that a factor distinct from TEF-1 binds specifically to Trex. Thus it appears that MCK transcription is regulated in skeletal muscles through a Trex-dependent pathway while Trex is not required for MCK expression in heart. This distinction could account partially for the difference in levels of muscle creatine kinase in these tissues.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/09/20 alle ore 00:35:29