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Titolo:
CHIRAL ANALYSIS OF 12-HYDROXYEICOSATETRAENOIC ACID FORMED BY CALF CORNEAL EPITHELIAL MICROSOMES
Autore:
CONNERS MS; STOLTZ RA; SCHWARTZMAN ML;
Indirizzi:
NEW YORK MED COLL,DEPT PHARMACOL VALHALLA NY 10595
Titolo Testata:
Journal of ocular pharmacology and therapeutics
fascicolo: 1, volume: 12, anno: 1996,
pagine: 19 - 26
SICI:
1080-7683(1996)12:1<19:CAO1AF>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
ARACHIDONIC-ACID; 12-HYDROXY-5,8,10,14-EICOSATETRAENOIC ACID; (12S)-HYDROXYEICOSATETRAENOIC ACID; 12(R)-HYDROXYEICOSATRIENOIC ACID; RABBIT CORNEA; METABOLISM; LIPOXYGENASE; CYTOCHROME-P-450; PLATELETS; RAT;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
34
Recensione:
Indirizzi per estratti:
Citazione:
M.S. Conners et al., "CHIRAL ANALYSIS OF 12-HYDROXYEICOSATETRAENOIC ACID FORMED BY CALF CORNEAL EPITHELIAL MICROSOMES", Journal of ocular pharmacology and therapeutics, 12(1), 1996, pp. 19-26

Abstract

The production of 12-hydroxyeicosatetraenoic acid by the corneal epithelium of several species has been extensively reported yet the controversy over the exclusive production of the (S) epimer (a lipoxygenase-derived metabolite) endures. Incubation of calf corneal epithelial microsomes (3 mg/ml) with arachidonic acid and NADPH resulted in the formation of 12-hydroxy-5,8,10,14-eicosatetraenoic acid (12-HETE). The synthesis of 12-HETE was inhibited by SKF-525A and clotrimazole, selective inhibitors of cytochrome P450 dependent activities, but not by indomethacin or BW-755C, inhibitors of cyclooxygenase and lipoxygenase activities, respectively. Chiral analysis revealed the presence of both enantiomers; however, the R isomer was the predominant one, i.e., 91+/-5% vs. 9+/-5% for the R and S enantiomers, respectively. Since the R enantiomer is the product of a cytochrome P450-mediated reaction, it suggests that the major metabolic activity in these microsomes is cytochrome P450-dependent and supports the claim for cytochrome P450 reactions in this ocular tissue.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/09/20 alle ore 18:31:49