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Titolo:
CHARACTERIZATION OF THE HUMAN DOPAMINE D-3 RECEPTOR EXPRESSED IN TRANSFECTED CELL-LINES
Autore:
MACKENZIE RG; VANLEEUWEN D; PUGSLEY TA; SHIH YH; DEMATTOS S; TANG L; TODD RD; OMALLEY KL;
Indirizzi:
PARKE DAVIS & CO,DEPT PHARMACOL,2800 PLYMOUTH RD ANN ARBOR MI 48106 WASHINGTON UNIV,SCH MED,DEPT ANAT & NEUROBIOL ST LOUIS MO 63110 WASHINGTON UNIV,SCH MED,DEPT PSYCHIAT & GENET ST LOUIS MO 63110
Titolo Testata:
European journal of pharmacology. Molecular pharmacology section
fascicolo: 1, volume: 266, anno: 1994,
pagine: 79 - 85
SICI:
0922-4106(1994)266:1<79:COTHDD>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
BETA-ADRENERGIC-RECEPTOR; ARACHIDONIC-ACID RELEASE; AGONIST; ACCUMULATION; STIMULATION; HYDROLYSIS; MEMBRANE; AFFINITY; CYCLASE; CLONING;
Keywords:
DOPAMINE D-3 RECEPTOR; DOPAMINE D-2 RECEPTOR; RECEPTOR BINDING; CAMP ACCUMULATION; ARACHIDONIC ACID RELEASE; INOSITOL PHOSPHATE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
23
Recensione:
Indirizzi per estratti:
Citazione:
R.G. Mackenzie et al., "CHARACTERIZATION OF THE HUMAN DOPAMINE D-3 RECEPTOR EXPRESSED IN TRANSFECTED CELL-LINES", European journal of pharmacology. Molecular pharmacology section, 266(1), 1994, pp. 79-85

Abstract

A full-length cDNA clone of the human dopamine D-3 receptor was obtained by the polymerase chain reaction (PCR) using reverse-transcribed RNA from human brain as the template. The cDNA was inserted into an expression vector which was then stably transfected into either Chinese hamster ovary (CHO), SK-N-MC human epithelioma or mouse CCL1.3 fibroblast cell lines. Post-transfection, the B-max for D-3 receptor expression was 1.9, 1.1 and 0.4 pmol/mg protein in the CHO-K1, SK-N-MC and CCL1.3 cell lines, respectively. The D-3 receptor expressed in CHO-K1 and CCL1.3 cells exhibited similar radioligand binding profiles, especially for the D-3-selective compound, 7-hydroxy-2-(di-n-propylamino)tetralin (7-OH-DPAT). Radioligand-binding competition curves of presumed D-3agonists were shifted to the right by the addition of guanine nucleotides and Na+ to the assay buffer. Presumed D-3-receptor agonists had no effect on cAMP accumulation in any of the D-3-transfected cell linesalthough cAMP accumulation was inhibited by dopamine D-2 receptor activation in D-2-transfected CHO and CCL1.3 cells and by activation of the exogenously expressed neuropeptide Y receptor in SK-N-MC cells. Also, D-3 receptor activation neither potentiated ATP-stimulated arachidonic acid release from CHO cells nor stimulated inositol phosphate production in CCL1.3-cells although both of these responses were elicited by D-2 agonists in D-2-transfected cells. We conclude that the signalling properties of the D-3 receptor differ from those of its closest homolog, the D-3 receptor.

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Documento generato il 05/07/20 alle ore 07:09:48