Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
EARLY CYTOPATHIC FEATURES IN RAT ISCHEMIA MODEL AND RECONSTRUCTION BYNEURAL GRAFT
Autore:
ONIZUKA K; FUKUDA A; KUNIMATSU M; KUMAZAKI M; SASAKI M; TAKAKU A; NISHINO H;
Indirizzi:
NAGOYA CITY UNIV,SCH MED,DEPT PHYSIOL,MIZUHO KU NAGOYA AICHI 467 JAPAN NAGOYA CITY UNIV,SCH MED,DEPT BIOCHEM,MIZUHO KU NAGOYA AICHI 467 JAPAN TOYAMA MED & PHARMACEUT UNIV,FAC MED,DEPT NEUROSURG SUGITANI TOYAMA 93001 JAPAN
Titolo Testata:
Experimental neurology
fascicolo: 2, volume: 137, anno: 1996,
pagine: 324 - 332
SICI:
0014-4886(1996)137:2<324:ECFIRI>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROTEIN-KINASE-C; HIPPOCAMPAL-NEURONS; CEREBRAL-ISCHEMIA; CELL-SUSPENSIONS; STRIATAL GRAFTS; DARK NEURONS; PROTEOLYSIS; DAMAGE; TRANSPLANTS; INJURY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
31
Recensione:
Indirizzi per estratti:
Citazione:
K. Onizuka et al., "EARLY CYTOPATHIC FEATURES IN RAT ISCHEMIA MODEL AND RECONSTRUCTION BYNEURAL GRAFT", Experimental neurology, 137(2), 1996, pp. 324-332

Abstract

Using a silver impregnation (argyrophil III) and immunohistochemistry, acute cytopathic features after cerebral ischemia were investigated. Additionally, functional recovery and interconnection between the host and graft was also explored after neural graft. Animals were embolized in unilateral middle cerebral artery for 1 h. Argyrophil III methoddemonstrated ''collapsed'' dark neurons in the striatum, cortex, reticular thalamus, amygdala, and hypothalamus on ischemic side. These neurons exhibited characteristic shrunken somata with corkscrew-like dendrites, suggesting changes in cytoskeletal protein. In the above mentioned areas, the loss of immunoreactivity for mu-calpain proenzyme and microtubule-associated protein 2 was also detected. Neural graft into the ischemic striatum was made 2 weeks after the ischemia paradigm. Thegrafted striatal cells were prepared from E15 fetuses to make cell suspension marked by rhodamine-labeled latex microspheres. Methamphetamine-evoked rotations were detected after ischemia. These motor alterations were reduced gradually but significantly at 8 weeks after the graft. Interconnection between the host and grafted cells was then studiedin a brain slice preparation after loading fura-2 AM. About 10% of grafted cells tested from rats that showed motor amelioration exhibited [Ca2+](i) increase to the electrical stimulation applied to the neighboring host tissue. Data indicate that, in the very early stage after ischemia, cytoskeletal damages, especially on microtubules, started andthis would lead to later infarct. The graft survived in the ischemic striatum having connections with the host, and this might be partly involved in the amelioration of motor function. (C) 1996 Academic Press,Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/07/20 alle ore 10:28:24