Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
SIMPLIFIED PLASMID RESCUE OF HOST SEQUENCES ADJACENT TO INTEGRATED PROVIRUSES
Autore:
KURDIHAIDAR B; FRIEDMANN T;
Indirizzi:
UNIV CALIF SAN DIEGO,SCH MED,CTR CANC LA JOLLA CA 92093 UNIV CALIF SAN DIEGO,SCH MED,CTR MOLEC GENET LA JOLLA CA 92093 UNIV CALIF SAN DIEGO,SCH MED,DEPT PEDIAT LA JOLLA CA 92093
Titolo Testata:
Gene
fascicolo: 2, volume: 168, anno: 1996,
pagine: 199 - 203
SICI:
0378-1119(1996)168:2<199:SPROHS>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
INSERTIONAL MUTAGENESIS; DNA; CONSTRUCTION; RETROVIRUS; CELLS; MOUSE; SITE; IDENTIFICATION; MUTATION; CANCER;
Keywords:
RETROVIRUS; MOLONEY MURINE LEUKEMIA VIRUS; MOMLV; INSERTIONAL MUTAGENESIS; SEQUENCE-TAGGED SITE; STS; PCR MAPPING; SINGLE CLEAVAGE SITE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
21
Recensione:
Indirizzi per estratti:
Citazione:
B. Kurdihaidar e T. Friedmann, "SIMPLIFIED PLASMID RESCUE OF HOST SEQUENCES ADJACENT TO INTEGRATED PROVIRUSES", Gene, 168(2), 1996, pp. 199-203

Abstract

We have previously described a Moloney murine leukemia retroviral (MoMLV) vector useful for the generation of anchored long-range maps of complex mammalian genomes. We now report the development of a modified vector carrying the ColE1 origin of replication and the chloramphenicol-resistance (Cm-R) gene to facilitate the recovery of genomic sequences adjacent to integrated proviruses. We demonstrate the utility of this new vector for the rescue in plasmid form of a 6-kb human fragment containing portions of an Alu element adjacent to the proviral 3'-LTR from an infected human primary fibroblast clone. We generated a sequence-tagged site (STS) from the derived sequence outside the Alu element, and used a somatic cell hybrid mapping panel to assign this STS to human chromosome 10 by a polymerase chain reaction-based assay. We suggest that this new Cm-R vector will be useful for recovering sequences of genes interrupted by proviral insertion, to generate directional maps with respect to the inserted provirus using the single cleavage sites within the vector, and to generate site-specific STS for defining and mapping the integration site.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 31/05/20 alle ore 07:21:14