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Titolo:
DEVELOPMENTAL HETEROGENEITY OF V-GAMMA-1.1 T-CELLS IN THE MOUSE-LIVER
Autore:
KODAIRA Y; YOKOMURO K; TANAKA S; MIYAZAKI JI; IKUTA K;
Indirizzi:
UNIV TOKYO,FAC MED,DEPT DIS RELATED GENE REGULAT RES SANDOZ,BUNKYO KU,HONGO 7-3-1 TOKYO 113 JAPAN UNIV TOKYO,FAC MED,DEPT DIS RELATED GENE REGULAT RES SANDOZ,BUNKYO KUTOKYO 113 JAPAN NIPPON MED COLL,DEPT MICROBIOL & IMMUNOL TOKYO JAPAN NIPPON MED COLL,DEPT SURG 2 TOKYO JAPAN
Titolo Testata:
Immunology
fascicolo: 2, volume: 87, anno: 1996,
pagine: 213 - 219
SICI:
0019-2805(1996)87:2<213:DHOVTI>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
RECEPTOR-GAMMA-DELTA; JUNCTIONAL SEQUENCES; N-REGIONS; STEM-CELLS; YOUNG MICE; GENES; DIVERSITY; LYMPHOCYTES; SUBSET; REARRANGEMENT;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
38
Recensione:
Indirizzi per estratti:
Citazione:
Y. Kodaira et al., "DEVELOPMENTAL HETEROGENEITY OF V-GAMMA-1.1 T-CELLS IN THE MOUSE-LIVER", Immunology, 87(2), 1996, pp. 213-219

Abstract

Modifications at V-(D)-J junctions increase the diversity of T-cell receptors (TCR). It has been shown that the levels of N-nucleotide insertion at the V-(D)-J junction in TCR transcripts are different betweenfetal and adult stages. To clarify developmental stages and pathways of gamma delta T cells in the liver, we analysed the nucleotide sequence of V gamma 1.1-J gamma 4, junctions of intra-hepatic lymphocytes (IHL), spleen cells and developing thymocytes from normal and athymic nude mice. The level of N-insertion increased in thymocytes during ontogeny. The percentage of V gamma 1.1-J gamma 4 transcripts with N-insertion was 3% at day 16 of gestation, 42% at newborn, and 89% at 7 weeks. Transcripts from normal IHL showed intermediate levels of N-insertionbetween those of newborn and adult thymocytes. In contrast the percentage of N-insertion in nude II-IL was 47%, and this value was comparable to that of newborn thymocytes. Among the transcripts of normal II-IL, the sequences common with nude IHL showed a newborn level of N-insertion (38%), and the remaining sequences showed an adult level (89%). These results suggested the possibility that V gamma 1.1-expressing T cells in II-IL might be a heterogeneous population consisting of the cells developed extrathymically as well as the cells developed intrathymically. The V gamma 1.1-J gamma 4 junctions from spleen cells showed less variability than those from IHL and adult thymocytes. It suggested that gamma delta T cells bearing specific V gamma 1.1 TCR develop and/or home in the spleen.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 21:43:57