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Titolo:
CYTOSKELETAL CHANGES IN CULTURED HUMAN FIBROBLASTS FOLLOWING EXPOSURETO 2,5-HEXANEDIONE
Autore:
PASSARIN MG; MONACO S; FERRARI S; GIANNINI C; RIZZUTO N; MORETTO G;
Indirizzi:
UNIV VERONA,POLICLIN BORGO ROMA,DIPARTIMENTO SCI NEUROL & VIS,SEZ NEUROL I-37134 VERONA ITALY UNIV VERONA,POLICLIN BORGO ROMA,DIPARTIMENTO SCI NEUROL & VIS,SEZ NEUROL I-37134 VERONA ITALY
Titolo Testata:
Neuropathology and applied neurobiology
fascicolo: 1, volume: 22, anno: 1996,
pagine: 60 - 67
SICI:
0305-1846(1996)22:1<60:CCICHF>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
INTERMEDIATE FILAMENTS; NEUROFILAMENT TRANSPORT; MOLECULAR PATHOGENESIS; COVALENT CROSSLINKING; VIMENTIN-FILAMENTS; HEXANE NEUROPATHY; NERVOUS-SYSTEM; INVITRO; AGGREGATION; PROTEINS;
Keywords:
CELL CULTURES; 2,5-HEXANEDIONE; INTERMEDIATE FILAMENTS; MICROTUBULES; CELL CYCLE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
26
Recensione:
Indirizzi per estratti:
Citazione:
M.G. Passarin et al., "CYTOSKELETAL CHANGES IN CULTURED HUMAN FIBROBLASTS FOLLOWING EXPOSURETO 2,5-HEXANEDIONE", Neuropathology and applied neurobiology, 22(1), 1996, pp. 60-67

Abstract

In the present study, the effects of the 2,5-hexanedione (HD) on the various cytoskeletal components of human eukaryotic cells were investigated. Primary cultures of human fibroblasts from three healthy donorswere exposed to 2.8 mM HD fur 14 days; unexposed cultures were used as controls. At different time intervals, cells were counted and the growth curves compared, After 14 days of treatment, the cultures were processed for immunoblotting, immunocytochemistry and ultrastructural studies. as compared to controls, morphological abnormalities induced byHD consisted of modification of the cell shape, formation of tangles composed by 10 nm filaments and cytoplasmic segregation of microtubules and membrane-bound organelles. By immunocytochemistry, the tangles of cytoplasmic filaments were stained by an antibody specific for vimentin. By immunoblotting, the anti-vimentin antibody recognized only bands of 50-60 kDa. Comparison of growth curves between treated and control fibroblasts clearly revealed an interference of HD with the cell cycle, The present results demonstrate that the cytotoxicity of HD is not restricted to intermediate filaments, but affects other cytoskeletalcomponents, such as microtubules, as suggested by the impairment of cell division cycle.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/07/20 alle ore 23:29:10