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Titolo:
PHOSPHORYLATION OF NEUROFILAMENT PROTEINS IN ISOLATED GOLDFISH MAUTHNER AXOPLASM
Autore:
RAABE TD; BITTNER GD;
Indirizzi:
UNIV TEXAS,DEPT ZOOL AUSTIN TX 78712 UNIV TEXAS,DEPT ZOOL AUSTIN TX 78712 UNIV TEXAS,DEPT PHARMACOL AUSTIN TX 78712 UNIV TEXAS,INST NEUROSCI AUSTIN TX 78712
Titolo Testata:
Journal of neurochemistry
fascicolo: 3, volume: 66, anno: 1996,
pagine: 1214 - 1221
SICI:
0022-3042(1996)66:3<1214:PONPII>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
SQUID GIANT-AXON; CASEIN KINASE-I; INTERMEDIATE FILAMENTS; SPINAL-CORD; SELECTIVE INHIBITOR; CDC2-LIKE KINASE; PROTEOLYSIS; SUBUNIT; INVIVO; POLYPEPTIDES;
Keywords:
NEUROFILAMENT; PHOSPHORYLATION; KINASE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
50
Recensione:
Indirizzi per estratti:
Citazione:
T.D. Raabe e G.D. Bittner, "PHOSPHORYLATION OF NEUROFILAMENT PROTEINS IN ISOLATED GOLDFISH MAUTHNER AXOPLASM", Journal of neurochemistry, 66(3), 1996, pp. 1214-1221

Abstract

The six neurofilament proteins (NFPs) in the goldfish Mauthner axon (M-axon) have molecular sizes of 235, 145, 123, 105, 80, and 60 kDa. Todetermine if NFPs in the M-axon are phosphorylated, isolated Mauthneraxoplasm (M-axoplasm) and a neurofilament-enriched extract (NFE) prepared from M-axoplasm were incubated with P-32, which resulted in the radiolabeling of NFPs as determined by their detection on autoradiograms. Kinase inhibitors directed against cyclic AMP-dependent kinases (PKAs) or cofactor-independent kinases significantly reduced the in vitrophosphorylation of NFPs in NFE, whereas inhibitors directed against protein kinase C did not significantly reduce the in vitro phosphorylation of NFPs in NFE. Experiments using two kinase inhibitors directed against different kinases significantly reduced the in vitro phosphorylation of NFPs in NFE to a greater extent than the reduction produced using any single kinase inhibitor. These data suggest that NFPs in the M-axon are phosphorylated and that the in vitro (and perhaps the in vivo) phosphorylation of NFPs is mediated by PKA and/or cofactor-independent kinases that copurify with NFPs.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/09/20 alle ore 12:04:43