Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
DISTANCE DISTRIBUTIONS FROM THE TYROSYL TO DISULFIDE RESIDUES IN THE OXYTOCIN AND [ARG(8)]-VASOPRESSIN MEASURED USING FREQUENCY-DOMAIN FLUORESCENCE RESONANCE ENERGY-TRANSFER
Autore:
SZMACINSKI H; WICZK W; FISHMAN MN; EIS PS; LAKOWICZ JR; JOHNSON ML;
Indirizzi:
UNIV MARYLAND,SCH MED,CTR FLUORESCENCE SPECT BALTIMORE MD 21201 UNIV MARYLAND,SCH MED,CTR FLUORESCENCE SPECT BALTIMORE MD 21201 UNIV MARYLAND,SCH MED,DEPT BIOL CHEM BALTIMORE MD 21201 UNIV VIRGINIA,DEPT PHARMACOL CHARLOTTESVILLE VA 22908
Titolo Testata:
European biophysics journal
fascicolo: 3, volume: 24, anno: 1996,
pagine: 185 - 193
SICI:
0175-7571(1996)24:3<185:DDFTTT>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
TIME-RESOLVED FLUORESCENCE; TO-END DIFFUSION; DECAY KINETICS; CONFORMATIONAL FLEXIBILITY; ROTAMER POPULATIONS; DEAMINO-OXYTOCIN; MODULATION DATA; TROPONIN-C; FLUOROMETRY; RESOLUTION;
Keywords:
FLUORESCENCE; ENERGY TRANSFER; OXYTOCIN; VASOPRESSIN; DISTANCE DISTRIBUTION; TIME-RESOLVED FLUORESCENCE; FREQUENCY-DOMAIN FLUORESCENCE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
36
Recensione:
Indirizzi per estratti:
Citazione:
H. Szmacinski et al., "DISTANCE DISTRIBUTIONS FROM THE TYROSYL TO DISULFIDE RESIDUES IN THE OXYTOCIN AND [ARG(8)]-VASOPRESSIN MEASURED USING FREQUENCY-DOMAIN FLUORESCENCE RESONANCE ENERGY-TRANSFER", European biophysics journal, 24(3), 1996, pp. 185-193

Abstract

We have examined the fluorescence intensity decays of oxytocin and [Arg(8)]-vasopressin resulting from the single tyrosyl residue in each peptide, and the intensity decay of the Asu(1,6)-analogues in which thedisulfide bridge is substituted by a CH2-CH2 bridge. Viscosity-dependent steady state and intensity decay measurements indicated that fluorescence resonance energy transfer (FRET) from tyrosyl phenol to the disulfide bridge is responsible for the decrease in fluorescence relative to the Asu-analogues. The frequency-domain phase and modulation datafor the tyrosyl donor were interpreted in terms of fluorescence resonance energy transfer (FRET) to the weakly absorbing disulfide bridge and a distribution of donor-to-acceptor distances. Energy transfer efficiencies were determined from both time-resolved and steady-state measurements. Fitting the frequency-domain phase and modulation data to a Gaussian distance distribution indicated that the average inter-chromophoric distance (R(av)) is similar in both compounds, R(av)=7.94 Angstrom for oxytocin and R(av) = 8.00 Angstrom for vasopressin. However, the width of the distance distribution is narrower for vasopression (hw=2.80 Angstrom) than for oxytocin (hw=3.58 A), which is consistent with restriction of the tyrosine phenol motion due to its stacking with the Phe(3) side chain of vasopressin. Finally, the recovered distance distribution functions are compared with histograms describing the distance between the chromophores during the course of long, in vacuo, molecular dynamics runs using the computer program CHARMm and the QUANTA 3.0 parameters.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 16:17:42