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Titolo:
IDENTIFICATION OF A REGION REQUIRED FOR SUBTYPE-SPECIFIC AGONIST-INDUCED SEQUESTRATION OF THE M2 MUSCARINIC ACETYLCHOLINE-RECEPTOR
Autore:
GOLDMAN PS; SCHLADOR ML; SHAPIRO RA; NATHANSON NM;
Indirizzi:
UNIV WASHINGTON,DEPT PHARMACOL,K536A HSB,BOX 357750 SEATTLE WA 98195 UNIV WASHINGTON,DEPT PHARMACOL SEATTLE WA 98195
Titolo Testata:
The Journal of biological chemistry
fascicolo: 8, volume: 271, anno: 1996,
pagine: 4215 - 4222
SICI:
0021-9258(1996)271:8<4215:IOARRF>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
3RD CYTOPLASMIC LOOP; G-PROTEIN; CHOLINERGIC RECEPTORS; CELLS; M1; DOMAIN; EXPRESSION; BINDING; M3-MUSCARINIC-RECEPTOR; DESENSITIZATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
43
Recensione:
Indirizzi per estratti:
Citazione:
P.S. Goldman et al., "IDENTIFICATION OF A REGION REQUIRED FOR SUBTYPE-SPECIFIC AGONIST-INDUCED SEQUESTRATION OF THE M2 MUSCARINIC ACETYLCHOLINE-RECEPTOR", The Journal of biological chemistry, 271(8), 1996, pp. 4215-4222

Abstract

When the m1 and m2 muscarinic acetylcholine receptors are transientlyexpressed in JEG-3 cells, the m2, but not the mi, receptor undergoes agonist-induced sequestration. Both receptors exhibit internalization when expressed in Y1 cells. These results suggest that the m1 and m2 receptors use distinct cellular mechanisms or pathways for agonist-induced internalization and that JEG-3 cells are deficient in the mechanism or pathway used by the m1 receptor. Transfection experiments with chimeric receptors indicate that the specificity for agonist-induced internalization for the m2 receptor lies in the carboxyl-terminal fifth of the receptor. The intracellular carboxyl-terminal tail of the m2 receptor is neither sufficient nor required for the m2-specific sequestration. Site-directed mutagenesis demonstrates that two amino acids in the carboxyl-terminal end of the third cytoplasmic loop of the m2 receptor are required for sequestration in JEG-3 cells. In addition, the sixth transmembrane domain, which is adjacent to this cytoplasmic domain, is also required. Thus, m2-specific agonist-induced sequestration requires sequences both in the carboxyl-terminal end of the third cytoplasmic loop and the adjacent transmembrane domain.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/11/20 alle ore 19:53:32