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Titolo:
FACTORS MODIFYING PROTECTIVE EFFECT OF ANTI-CD18 ANTIBODIES ON MYOCARDIAL REPERFUSION INJURY IN DOGS
Autore:
PEREZ RG; ARAI M; RICHARDSON C; DIPAULA A; SIU C; MATSUMOTO N; HILDRETH JEK; MARISCALCO MM; SMITH CW; BECKER LC;
Indirizzi:
JOHNS HOPKINS UNIV HOSP,600 N WOLFE ST,HALSTED 500 BALTIMORE MD 21287 JOHNS HOPKINS MED INST,DEPT MED,DIV CARDIOL BALTIMORE MD 21205 JOHNS HOPKINS MED INST,DEPT PHARMACOL BALTIMORE MD 21205 BAYLOR COLL MED,DEPT PEDIAT,SECT LEUKOCYTE BIOL,SPEROS P MARTEL LAB HOUSTON TX 77030
Titolo Testata:
American journal of physiology. Heart and circulatory physiology
fascicolo: 1, volume: 39, anno: 1996,
pagine: 53 - 64
SICI:
0363-6135(1996)39:1<53:FMPEOA>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
INTERCELLULAR-ADHESION MOLECULE-1; INFARCT SIZE; NEUTROPHIL ACCUMULATION; MONOCLONAL-ANTIBODY; CHEMOTACTIC FACTORS; HYDROGEN-PEROXIDE; BETA-SUBUNIT; CANINE HEART; ISCHEMIA; ADHERENCE;
Keywords:
MYOCARDIAL INFARCTION; NEUTROPHILS; ADHESION PROTEINS; MONOCLONAL ANTIBODIES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
42
Recensione:
Indirizzi per estratti:
Citazione:
R.G. Perez et al., "FACTORS MODIFYING PROTECTIVE EFFECT OF ANTI-CD18 ANTIBODIES ON MYOCARDIAL REPERFUSION INJURY IN DOGS", American journal of physiology. Heart and circulatory physiology, 39(1), 1996, pp. 53-64

Abstract

Anti-CD18 monoclonal antibodies (MAb) have demonstrated variable protection against neutrophil (PMN)-mediated myocardial reperfusion injury. To identify factors contributing to this variability, open-chest dogs underwent coronary artery occlusion for 90 min followed by reperfusion for 3.5 h. Ten minutes before reperfusion the dogs received saline (n = 18) or one of three anti-CD18 MAb: MHM.23, R15.7, or PLM-2 (2, 1,and 1 mg/kg and n = 19, 8, and 4, respectively). Collateral flow was measured with radioactive microspheres, area at risk was assessed withmonastral blue dye, and infarct size was measured postmortem by triphenyltetrazolium chloride. In vitro, all three MAb bound to canine PMNs, but only MHM.23 and R15.7 inhibited their adherence to keyhole limpet hemocyanin-coated plastic. In vivo, only MHM.23 and R15.7 significantly reduced infarct size after adjusting for the effect of collateral flow. MHM.23 afforded protection in dogs with moderate ischemia (epicardial collateral flow >0.1 ml . min(-1). g(-1), infarct size reduced 46%) but not in dogs with more severe ischemia. Only R15.7 was effective in dogs with severe ischemia. Although MHM.23 and R15.7 produced similar inhibition of tissue PMN accumulation, as reflected by myeloperoxidase activity, R15.7 markedly inhibited H2O2 production by PMNs afterexposure to platelet-activating factor, whereas MHM.23 had only a minimal effect. The effectiveness of different anti-CD18 MAb in preventing reperfusion injury appears to be 1) highly dependent on the specificanti-CD18 MAb employed, 2) predicted only partially by in vitro binding to PMNs, static in vitro tests of PMN adherence, or the extent of inhibition of PMN accumulation in vivo, 3) related more to their ability to inhibit oxidant release from activated PMNs, and 4) strongly influenced by the severity of myocardial ischemia before reperfusion.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 15/01/21 alle ore 21:59:55