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Titolo:
UNUSUAL TRIGONAL-PLANAR COPPER CONFIGURATION REVEALED IN THE ATOMIC-STRUCTURE OF YEAST COPPER-ZINC SUPEROXIDE-DISMUTASE
Autore:
OGIHARA NL; PARGE HE; HART PJ; WEISS MS; GOTO JJ; CRANE BR; TSANG J; SLATER K; ROE JA; VALENTINE JS; EISENBERG D; TAINER JA;
Indirizzi:
UNIV CALIF LOS ANGELES,DEPT CHEM & BIOCHEM,BOX 951569 LOS ANGELES CA 90095 UNIV CALIF LOS ANGELES,DEPT CHEM & BIOCHEM LOS ANGELES CA 90095 UNIV CALIF LOS ANGELES,US DOE,LAB STRUCT BIOL & MOLEC MED LOS ANGELESCA 90095 SCRIPPS CLIN & RES FDN,RES INST,DEPT MOLEC BIOL LA JOLLA CA 92037 LOYOLA MARYMOUNT UNIV,DEPT CHEM & BIOCHEM LOS ANGELES CA 90045
Titolo Testata:
Biochemistry
fascicolo: 7, volume: 35, anno: 1996,
pagine: 2316 - 2321
SICI:
0006-2960(1996)35:7<2316:UTCCRI>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
SUBSTITUTED DERIVATIVES; PULSE-RADIOLYSIS; MECHANISM; RESONANCE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
30
Recensione:
Indirizzi per estratti:
Citazione:
N.L. Ogihara et al., "UNUSUAL TRIGONAL-PLANAR COPPER CONFIGURATION REVEALED IN THE ATOMIC-STRUCTURE OF YEAST COPPER-ZINC SUPEROXIDE-DISMUTASE", Biochemistry, 35(7), 1996, pp. 2316-2321

Abstract

The three-dimensional structure of yeast copper-zinc superoxide dismutase (CuZnSOD) has been determined in a new crystal form in space group R32 and refined against X-ray diffraction data using difference Fourier and restrained crystallographic refinement techniques, The unexpected result is that the copper ion has moved approximately 1 Angstrom from its position in previously reported CuZnSOD models, the copper-imidazolate bridge is broken, and a roughly trigonal planar ligand geometry characteristic of Cu-I rather than Cu-II is revealed. Final R values for the two nearly identical room temperature structures are 18.6% for all 19 149 reflections in the 10.0-1.7 Angstrom resolution range and 18.2% for 17 682 reflections (F > 2 sigma) in the 10.0-1.73 Angstromresolution range. A third structure has been determined using X-ray data collected at -180 degrees C. The final R value for this structure is 19.0% (R(free) = 22.9%) for all 24 356 reflections in the 10.0-1.55Angstrom resolution range, Virtually no change in the positions of the ligands to the zinc center is observed in these models. The origin of the broken bridge and altered Cu-ligand geometry is discussed.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/09/20 alle ore 03:02:40