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Titolo:
CASEIN KINASE-II IS REQUIRED FOR EFFICIENT TRANSCRIPTION BY RNA-POLYMERASE-III
Autore:
HOCKMAN DJ; SCHULTZ MC;
Indirizzi:
UNIV ALBERTA,DEPT BIOCHEM EDMONTON AB T6G 2H7 CANADA UNIV ALBERTA,DEPT BIOCHEM EDMONTON AB T6G 2H7 CANADA
Titolo Testata:
Molecular and cellular biology
fascicolo: 3, volume: 16, anno: 1996,
pagine: 892 - 898
SICI:
0270-7306(1996)16:3<892:CKIRFE>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
TATA-BINDING-PROTEIN; SACCHAROMYCES-CEREVISIAE; CELLULAR-REGULATION; RIBOSOMAL-RNA; YEAST; SUBUNIT; GENE; PHOSPHORYLATION; INITIATION; INVIVO;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
61
Recensione:
Indirizzi per estratti:
Citazione:
D.J. Hockman e M.C. Schultz, "CASEIN KINASE-II IS REQUIRED FOR EFFICIENT TRANSCRIPTION BY RNA-POLYMERASE-III", Molecular and cellular biology, 16(3), 1996, pp. 892-898

Abstract

Casein kinase II (CKII) is a ubiquitous and highly conserved serine/threonine protein kinase found in the nucleus and cytoplasm of most cells, Using a combined biochemical and genetic approach in the yeast Saccharomyces cevevisiae, we assessed the role of CKII in specific transcription by RNA polymerases I, II, and III. CKII is not required for basal transcription by RNA polymerases I and II but is important for polymerase III transcription, Polymerase III transcription is high in extracts with normal CKII activity but low in extracts from a temperature-sensitive mutant that has decreased CKII activity due to a lesion in the enzyme's catalytic alpha ' subunit, Polymerase III transcription of 5S rRNA and tRNA templates in the temperature-sensitive extract is rescued by purified, wild-type CKII, An inhibitor of CKII represses polymerase III transcription in wild-type extract, and this repression ispartly overcome by supplementing reaction mixtures with active CKII. Finally, we show that polymerase III transcription in vivo is impairedwhen CKII is inactivated, Our results demonstrate that CKII, an oncogenic protein kinase previously implicated in cell cycle and growth control, is required for high-level transcription by RNA polymerase III.

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Documento generato il 30/11/20 alle ore 10:11:29