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Titolo:
EXPRESSION OF XENOBIOTIC-METABOLIZING CYTOCHROME-P450 FORMS IN HUMAN FULL-TERM PLACENTA
Autore:
HAKKOLA J; PASANEN M; HUKKANEN J; PELKONEN O; MAENPAA J; EDWARDS RJ; BOOBIS AR; RAUNIO H;
Indirizzi:
UNIV OULU,DEPT PHARMACOL & TOXICOL,KAJAANINTIE 52D SF-90220 OULU FINLAND UNIV KUOPIO,DEPT PHARMACOL & TOXICOL SF-90721 KUOPIO FINLAND ROYAL POSTGRAD MED SCH,DEPT CLIN PHARMACOL LONDON W12 0NN ENGLAND
Titolo Testata:
Biochemical pharmacology
fascicolo: 4, volume: 51, anno: 1996,
pagine: 403 - 411
SICI:
0006-2952(1996)51:4<403:EOXCFI>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
CDNA-DIRECTED EXPRESSION; AMINO-ACID SEQUENCE; HUMAN-LIVER; GENE SUBFAMILY; HUMAN-FETAL; DIFFERENTIAL EXPRESSION; CIGARETTE-SMOKING; AH-RECEPTOR; HUMAN-LUNG; IDENTIFICATION;
Keywords:
CYTOCHROME P450; MATERNAL-FETAL EXCHANGE; SMOKING; POLYMERASE CHAIN REACTION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
65
Recensione:
Indirizzi per estratti:
Citazione:
J. Hakkola et al., "EXPRESSION OF XENOBIOTIC-METABOLIZING CYTOCHROME-P450 FORMS IN HUMAN FULL-TERM PLACENTA", Biochemical pharmacology, 51(4), 1996, pp. 403-411

Abstract

The expression of individual xenobiotic-metabolizing cytochrome P450 (CYP) genes in human placenta was studied at the mRNA level by reversetranscriptase-polymerase chain reaction (RT-PCR). mRNAs of CYP1A1, CYP2E1, CYP2F1, CYP3A3/4, CYP3A5, and CYP4B1 were detected by RT-PCR, and CYP1A2, CYP2A6/7, CYP2B6/7, CYP2C8-19, CYP2D6, and CYP3A7 were not detected. Several enzyme activity assays and immunoblots were used to further characterize expression of forms producing detectable mRNA transcripts. The catalytic activities of 7-ethoxycoumarin O-deethylase (ECOD), 7-ethoxyresorufin O-deethylase (EROD) and aryl hydrocarbon hydroxylase (AHH) were substantially increased in response to maternal cigarette smoking, and paralleled the amount of CYP1A1 mRNA and protein. Aromatase activities were slightly lower in placentas exposed to cigarette smoke compared with nonexposed placentas. These data show that several xenobiotic-metabolizing CYP genes are expressed in human placenta at a low level. The significance of such low-level expression is unknown, but it may have local physiological or toxic consequences.

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Documento generato il 31/03/20 alle ore 16:07:56