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Titolo:
MOLECULAR-CLONING AND PHOTOPERIOD-REGULATED EXPRESSION OF GIBBERELLIN20-OXIDASE FROM THE LONG-DAY PLANT SPINACH
Autore:
WU KQ; LI L; GAGE DA; ZEEVAART JAD;
Indirizzi:
MICHIGAN STATE UNIV,DEPT ENERGY,PLANT RES LAB E LANSING MI 48824 MICHIGAN STATE UNIV,DEPT ENERGY,PLANT RES LAB E LANSING MI 48824 MICHIGAN STATE UNIV,DEPT BIOCHEM E LANSING MI 48824
Titolo Testata:
Plant physiology
fascicolo: 2, volume: 110, anno: 1996,
pagine: 547 - 554
SICI:
0032-0889(1996)110:2<547:MAPEOG>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
ENDOGENOUS GIBBERELLINS; STEM ELONGATION; SILENE-ARMERIA; SHOOT TIPS; METABOLISM; LEAVES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
24
Recensione:
Indirizzi per estratti:
Citazione:
K.Q. Wu et al., "MOLECULAR-CLONING AND PHOTOPERIOD-REGULATED EXPRESSION OF GIBBERELLIN20-OXIDASE FROM THE LONG-DAY PLANT SPINACH", Plant physiology, 110(2), 1996, pp. 547-554

Abstract

Spinach (Spinacia oleracea L.) is a long-day (LD) rosette plant in which stem growth under LD conditions is mediated by gibberellins (GAs). Major control points in spinach are the later steps of sequential oxidation and elimination of C-20 of C-20-GAs. Degenerate oligonucleotideprimers were used to obtain a polymerase chain reaction product from spinach genomic DNA that has a high homology with CA 20-oxidase cDNAs from Cucurbita maxima L. and Arabidopsis thaliana Heynh. This polymerase chain reaction product was used as a probe to isolate a full-lengthcDNA clone with an open reading frame encoding a putative 43-kD protein of 374 amino acid residues. When this cDNA clone was expressed in Escherichia coli, the fusion protein catalyzed the biosynthetic sequence GA(53)--> GA(44)--> GA(19)--> GA(20) and GA(19)--> GA(17). This establishes that in spinach a single protein catalyzes the oxidation and elimination of C-20. Transfer of spinach plants from short day (SD) to LD conditions caused an increase in the level of all GAs of the early-13-hydroxylation pathway, except GA(53), with GA(20), GA(1), and GA(8)showing the largest increases. Northern blot analysis indicated that the level of GA 20-oxidase mRNA was higher in plants in LD than in SD conditions, with highest level of expression in the shoot tips and elongating stems. This expression pattern of GA 20-oxidase is consistent with the different levels of GA(20), GA(1), and GA(8) found in spinachplants grown in SD and LD conditions.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 11:51:30