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Titolo:
ROLE OF TYROSINE KINASES, PROTEIN-KINASE-C, AND PROTEIN-KINASE-A IN THE REGULATION OF INTERFERON-ALPHA PRODUCTION INDUCED BY HERPES-SIMPLEXVIRUS TYPE-1
Autore:
LI QH; FELDMAN M; HARMON C; FITZGERALDBOCARSLY P;
Indirizzi:
UMDNJ,NEW JERSEY MED SCH,DEPT PATHOL & LAB MED NEWARK NJ 07103 UMDNJ,NEW JERSEY MED SCH,DEPT PATHOL & LAB MED NEWARK NJ 07103 UMDNJ,NEW JERSEY MED SCH,GRAD SCH BIOMED SCI NEWARK NJ 07103
Titolo Testata:
Journal of interferon & cytokine research
fascicolo: 2, volume: 16, anno: 1996,
pagine: 109 - 118
SICI:
1079-9907(1996)16:2<109:ROTKPA>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN-IMMUNODEFICIENCY-VIRUS; BLOOD MONONUCLEAR LEUKOCYTES; T-CELL ACTIVATION; IFN-BETA GENES; SIGNAL-TRANSDUCTION; B-CELLS; INHIBITION; INDUCTION; PHOSPHORYLATION; STIMULATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
48
Recensione:
Indirizzi per estratti:
Citazione:
Q.H. Li et al., "ROLE OF TYROSINE KINASES, PROTEIN-KINASE-C, AND PROTEIN-KINASE-A IN THE REGULATION OF INTERFERON-ALPHA PRODUCTION INDUCED BY HERPES-SIMPLEXVIRUS TYPE-1", Journal of interferon & cytokine research, 16(2), 1996, pp. 109-118

Abstract

Herpes simplex virus 1 (HSV-1) is able to induce interferon-a production by natural IFN-alpha-producing cells, In this study, signal transduction in this process was examined, It was found that sequestering ofcalcium by EGTA abolished IFN-alpha-induction by HSV-infected cells, Stimulation of human PBMC by HSV-1-infected fibroblasts resulted in the production of inositol triphosphate (InsP(3)) and tyrosine phosphorylation of cellular proteins, The protein kinase C inhibitor, H7, and the tyrosine kinase inhibitor, herbimycin A, were able to suppress IFN-alpha gene expression as determined by IFN bioassay and RT-PCR. An IFN-alpha-specific ELISpot assay revealed that herbimycin A and H7 remarkably decreased the number of IFN-alpha-producing cells, PMA or calciumionophore A23187 alone did not increase IFN-alpha production, However, PMA in conjugation with ionophores increased IFN-alpha production asearly as 2 h. HA1004 and 2',5'-dideoxyadenosine, which are potent inhibitors of PKA pathway, had no effect on IFN-alpha production. In contrast, BrcAMP, a specific PKA activator, inhibited the IFN-alpha secretion and number of IFN-alpha-producing cells and to a lesser extent reduced the level of IFN-alpha mRNA. Our results indicate that protein kinase C, tyrosine kinases, and protein kinase A are involved in the regulation of IFN-alpha production in response to HSV-1.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/09/20 alle ore 00:20:18