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Titolo:
CYTOKINES DECREASE APOLIPOPROTEIN ACCUMULATION IN MEDIUM FROM HEP G2 CELLS
Autore:
ETTINGER WH; VARMA VK; SORCITHOMAS M; PARKS JS; SIGMON RC; SMITH TK; VERDERY PB;
Indirizzi:
MED CTR BLVD WINSTON SALEM NC 27157 WAKE FOREST UNIV,BOWMAN GRAY SCH MED,DEPT INTERNAL MED WINSTON SALEM NC 27103 WAKE FOREST UNIV,BOWMAN GRAY SCH MED,DEPT COMPARAT MED WINSTON SALEM NC 27103
Titolo Testata:
Arteriosclerosis and thrombosis
fascicolo: 1, volume: 14, anno: 1994,
pagine: 8 - 13
SICI:
1049-8834(1994)14:1<8:CDAAIM>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
TUMOR-NECROSIS-FACTOR; LECITHIN-CHOLESTEROL ACYLTRANSFERASE; DENSITY LIPOPROTEIN RECEPTOR; MESSENGER-RNA ABUNDANCE; A-I; FACTOR-ALPHA; HEPATIC LIPOGENESIS; CYNOMOLGUS MONKEYS; LIPID-METABOLISM; PLASMA-PROTEINS;
Keywords:
CYTOKINES; APOLIPOPROTEINS; HEP G2 CELLS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
54
Recensione:
Indirizzi per estratti:
Citazione:
W.H. Ettinger et al., "CYTOKINES DECREASE APOLIPOPROTEIN ACCUMULATION IN MEDIUM FROM HEP G2 CELLS", Arteriosclerosis and thrombosis, 14(1), 1994, pp. 8-13

Abstract

Cytokines, important biochemical mediators of inflammation, cause a rapid fall in the plasma concentration of cholesterol in vivo. One mechanism by which cytokines may cause acquired hypocholesterolemia is by decreasing the hepatic synthesis and secretion of apolipoproteins. To test this hypothesis, we incubated Hep G2 cells with human recombinanttumor necrosis factor-alpha, interleukin-1 beta and interleukin-6. Each of the cytokines resulted in a dose-related reduction in the concentrations of apolipoprotein (ape) A-I, apoB, and lecithin: cholesterol acyltransferase (LCAT) activity in the medium after 24 hours of incubation. The effect of cytokines on apolipoprotein accumulation was not affected by preincubation of Hep G2 cells with fatty acids. Cytokines decreased the concentration of cellular apoA-I mRNA in a dose-related fashion but did not affect cellular concentrations of apoB mRNA. The concentrations of triglyceride and cholesterol were also reduced in the medium of cells incubated with cytokines. Total cell sterol synthesis rates were calculated by [C-14]acetate incorporation. Cells incubated with interleukin-6 had a 31% increase in sterol synthesis rate but a 41% decrease in sterol secretion. These data suggest that these cytokines can decrease the hepatic synthesis and/or secretion of apolipoproteins and that this may explain, in part, the acquired hypocholesterolemia seen during acute and chronic inflammation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/11/20 alle ore 14:18:50