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Titolo:
GLYCOSAMINOGLYCANS MODULATE CELL-MATRIX INTERACTIONS OF HUMAN FIBROBLASTS AND ENDOTHELIAL-CELLS IN-VITRO
Autore:
SCHAEFER T; ROUX M; STUHLSATZ HW; HERKEN R; COULOMB B; KRIEG T; SMOLA H;
Indirizzi:
UNIV COLOGNE,DEPT DERMATOL,JOSEPH STELZMANN STR 9 D-50924 COLOGNE GERMANY UNIV COLOGNE,DEPT DERMATOL D-50924 COLOGNE GERMANY RHEIN WESTFAL TH AACHEN,DEPT CLIN CHEM D-52057 AACHEN GERMANY UNIV GOTTINGEN,DEPT ANAT D-37075 GOTTINGEN GERMANY HOP HENRI MONDOR,INSERM,U312,DERMATOL LAB F-94010 CRETEIL FRANCE
Titolo Testata:
Journal of Cell Science
, volume: 109, anno: 1996,
parte:, 2
pagine: 479 - 488
SICI:
0021-9533(1996)109:<479:GMCIOH>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
CHONDROITIN SULFATE PROTEOGLYCAN; CAPILLARY-LIKE STRUCTURES; COLLAGEN LATTICES; MOLECULAR-CLONING; CONTRACTION; MEMBRANE; INTEGRIN; INVITRO; LAMININ; DIFFERENTIATION;
Keywords:
CELL-MATRIX INTERACTION; GLYCOSAMINOGLYCAN; COLLAGEN; FIBROBLAST; ENDOTHELIAL CELL;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
41
Recensione:
Indirizzi per estratti:
Citazione:
T. Schaefer et al., "GLYCOSAMINOGLYCANS MODULATE CELL-MATRIX INTERACTIONS OF HUMAN FIBROBLASTS AND ENDOTHELIAL-CELLS IN-VITRO", Journal of Cell Science, 109, 1996, pp. 479-488

Abstract

Contact of various cells with extracellular matrix molecules modulates their cellular functions and phenotype, Most investigations have employed dishes coated with purified matrix constituents or plain collagen I lattices omitting the effects of other important matrix componentssuch as proteoglycans. In this study we analyze the effect of purified glycosaminoglycans (GAGs) on human fibroblasts and human umbilical vein endothelial cells (HUVEC) embedded within collagen I/III lattices. HUVEC contracted collagen I/III gels far less efficiently than fibroblasts and addition of heparan sulfate and heparin almost completely inhibited contraction, In collagen gels HUVEC down-regulated collagenasemRNA while increasing collagen I,IV mRNA expression. Addition of heparin and heparan sulfate reversed the collagen IV mRNA induction whereas hyaluronic acid and chondroitin sulfate enhanced fibronectin and collagenase transcripts, Fibroblasts readily contracted collagen gels, and mRNA levels for fibronectin, collagenase and interleukin-6 were stimulated. Gel contraction was mostly unaffected by the different glycosaminoglycans. Fibroblasts responded to the addition of dermatan sulfate, heparan sulfate and heparin with a decrease in fibronectin, collagenase and interleukin-6 mRNA. Binding studies revealed saturable bindingsites on fibroblasts and HUVEC for S-35-labelled heparin, demonstrating specificity for heparin and heparan sulfate over other GAGs in competition experiments. This study implies that glycosaminoglycans participate in cell-matrix interactions by effectively modulating the cellular phenotype via high affinity binding sites.

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Documento generato il 03/07/20 alle ore 00:57:40