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Titolo:
DIFFERENTIAL EXPRESSION OF EPITHELIAL SODIUM-CHANNEL SUBUNIT MESSENGER-RNAS IN RAT SKIN
Autore:
ROUDIERPUJOL C; ROCHAT A; ESCOUBET B; EUGENE E; BARRANDON Y; BONVALET JP; FARMAN N;
Indirizzi:
UNIV PARIS 07,FAC MED XAVIER BICHAT,INST FEDERAT RECH CELLULES EPITHELIALES,INSERM U246 F-75870 PARIS 18 FRANCE UNIV PARIS 07,FAC MED XAVIER BICHAT,INST FEDERAT RECH CELLULES EPITHELIALES,INSERM U246 F-75870 PARIS 18 FRANCE ECOLE NORMALE SUPER,DEPT BIOL,LAB DIFFERENCIAT EPITHELIALE F-75005 PARIS FRANCE
Titolo Testata:
Journal of Cell Science
, volume: 109, anno: 1996,
parte:, 2
pagine: 379 - 385
SICI:
0021-9533(1996)109:<379:DEOESS>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
SWEAT GLAND-FUNCTION; TRANSPORT INHIBITORS; NA+ CHANNEL; CELLS; LOCALIZATION; BINDING;
Keywords:
IN SITU HYBRIDIZATION; RNASE PROTECTION; SWEAT GLAND; HAIR FOLLICLE; EPIDERMIS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
28
Recensione:
Indirizzi per estratti:
Citazione:
C. Roudierpujol et al., "DIFFERENTIAL EXPRESSION OF EPITHELIAL SODIUM-CHANNEL SUBUNIT MESSENGER-RNAS IN RAT SKIN", Journal of Cell Science, 109, 1996, pp. 379-385

Abstract

Three subunits (alpha,beta,gamma) of the amiloride-sensitive epithelial sodium channel have been recently characterized. The channel subunits have significant homologies with the Caenorhabditis elegans mec-4, mec-10 and deg-P genes, which are;involved in control of cell volume and mecano-transduction. These subunits are coexpressed at equivalent levels in the renal collecting duct and the distal colon epithelium which are high resistance sodium transporting epithelia. We have investigated whether these subunits were expressed, at the mRNA level, in transporting as well as non transporting epithelial cells of rat skin. In full-thickness abdominal skin only alpha and gamma subunit mRNAs were detected, while all three subunit mRNAs were present in sole skin, as demonstrated by RNase-protection assay. Furthermore, the level of expression of each subunit varied with the epithelial cell type as demonstrated by in situ hybridization: epidermal and follicular keratinocytesexpress mostly alpha and gamma subunits (while beta was low); a prevalence of beta and gamma was observed in sweat glands. Thus, it appeared that two out of the three subunit mRNAs predominated in each epithelial structure. In addition, mRNAs of the alpha,beta and gamma subunitsof the amiloride-sensitive sodium channel were expressed at a higher level in large suprabasal epidermal keratinocytes (which undergo terminal differentiation) than in small proliferative basal keratinocytes.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/11/20 alle ore 22:05:21