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Titolo:
UBIQUITOUS NUCLEAR PROTEINS BIND TO 5' UPSTREAM REGION OF MAJOR KUNITZ CHYMOTRYPSIN INHIBITOR GENE IN WINGED BEAN
Autore:
HABU Y; SAKATA Y; FUKASAWA K; OHNO T;
Indirizzi:
HOKKAIDO UNIV,FAC AGR,DEPT APPL BIOSCI SAPPORO HOKKAIDO 060 JAPAN
Titolo Testata:
Plant molecular biology
fascicolo: 6, volume: 23, anno: 1993,
pagine: 1139 - 1150
SICI:
0167-4412(1993)23:6<1139:UNPBT5>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
BETA-CONGLYCININ GENES; TETRAGONOLOBUS L DC; FACTORS INTERACT; TRANSCRIPTIONAL REGULATION; CHROMOSOMAL-PROTEINS; STRUCTURAL FEATURES; NEGATIVE REGULATOR; A/T-RICH; DNA; SEQUENCE;
Keywords:
DNA-BINDING PROTEINS; HIGH MOBILITY GROUP PROTEINS; HMG PROTEINS; KUNITZ PROTEINASE INHIBITOR; SEED STORAGE PROTEIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
36
Recensione:
Indirizzi per estratti:
Citazione:
Y. Habu et al., "UBIQUITOUS NUCLEAR PROTEINS BIND TO 5' UPSTREAM REGION OF MAJOR KUNITZ CHYMOTRYPSIN INHIBITOR GENE IN WINGED BEAN", Plant molecular biology, 23(6), 1993, pp. 1139-1150

Abstract

Winged bean Kunitz chymotrypsin inhibitor (WCI) accumulates abundantly in seeds and tuberous roots of winged bean plant. In seeds, the WCI mRNA is observed transiently during seed maturation period. The WCI isencoded by a multigene family and the major WCI (WCI-3) is encoded bytwo nearly identical genes (WCI-3a and WCI-3b genes), in which nucleotide sequences in the 1.1 kb 5' flanking regions are about 99% homologous to each other and the transcribed regions are completely identical. Here we report the detection of two types of nuclear proteins which bind to the multiple sites in the 5' upstream region of the WCI-3a gene. One of the proteins, band 1-forming protein, also bound to cauliflower mosaic virus 35S (CaMV35S) promoter, but another protein, band 3-forming protein, did not. DNaseI footprinting analysis showed that these proteins bound to AT-rich upstream regions in the WCI-3a gene. Addition of poly(dA-dT)-poly(dA-dT) to the binding reaction inhibited the formation of the retarded bands, while poly(dI-dC)-poly(dI-dC) did not. In various organs and throughout seed maturation period, proteins with invariable binding specificities were detected, and these binding proteins met some operational criteria for high-mobility-group (HMG) proteins. These results suggest that leguminous seed AT-binding proteins reported on several seed storage protein genes may be HMG-like proteins which are present ubiquitously in plant organs.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/11/20 alle ore 20:14:05