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Titolo:
DIFFERENTIAL SECRETION OF DIMERIC INHIBIN IN CULTURED LUTEINIZED GRANULOSA-CELLS AS A FUNCTION OF OVARIAN RESERVE
Autore:
SEIFER DB; GARDINER AC; LAMBERTMESSERLIAN G; SCHNEYER AL;
Indirizzi:
BROWN UNIV,SCH MED,WOMEN & INFANTS HOSP,DEPT OBSTET & GYNECOL,DIV REPROD ENDOCRINOL PROVIDENCE RI 02905 MASSACHUSETTS GEN HOSP,NATL CTR INFERTIL RES BOSTON MA 02114
Titolo Testata:
The Journal of clinical endocrinology and metabolism
fascicolo: 2, volume: 81, anno: 1996,
pagine: 736 - 739
SICI:
0021-972X(1996)81:2<736:DSODII>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
FOLLICLE-STIMULATING-HORMONE; AGE; WOMEN; HYPERSTIMULATION; GONADOTROPIN; ESTRADIOL; PROTEINS; LEVEL; CYCLE; FLUID;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
17
Recensione:
Indirizzi per estratti:
Citazione:
D.B. Seifer et al., "DIFFERENTIAL SECRETION OF DIMERIC INHIBIN IN CULTURED LUTEINIZED GRANULOSA-CELLS AS A FUNCTION OF OVARIAN RESERVE", The Journal of clinical endocrinology and metabolism, 81(2), 1996, pp. 736-739

Abstract

Day 3 serum FSH is an indirect assessment of ovarian reserve and has been shown to be prognostic of outcome in ovulation induction and assisted reproductive technology programs. The precise physiologic basis for day 3 serum FSH screening is unknown. We tested the hypothesis thatdimeric inhibin is differentially secreted from luteinized granulosa cells collected from women in preparation for in vitro fertilization with low vs. high day 3 serum FSH levels. This prospective study consisted of luteinized granulosa cells harvested from 7 women with low day 3 serum FSH levels (less than or equal to 6 IU/L) and from 8 women with high FSH levels (greater than or equal to 10 IU/L) in preparation for in vitro fertilization-embryo transfer. Following retrieval, cells were isolated and then pooled within each individual patient and platedat 50,000 cells per well. Media was removed at 24 and 48 h and analyzed for dimeric and total inhibin concentrations by immunoassay as wellas estradiol and progesterone concentrations by RIA. Dimeric inhibin was secreted at 2-fold higher concentrations in the low FSH group 43.2pg/ml (30.8-60.6) (geometric mean and 95% confidence interval) compared with the high FSH group, 21.0 pg/ml (15.0-29.6), P < 0.004. Total inhibin was secreted at 1.8-fold higher concentrations in the low FSH group, 1148.2 pg/ml (931.1-1415.8) compared with the high FSH group, 639.7 pg/ml (428.5-955.0), P < 0.013. No significant differences in either estradiol or progesterone concentrations were noted. These data suggest that day 3 serum FSH is an indirect bioassay of dimeric inhibin production at the granulosa cell level. Thus, these data provide a potential physiological basis for day 3 serum FSH screening in ovulation induction and ART programs.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 18:05:11