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Titolo:
INSULIN-RECEPTOR MEDIATES INHIBITORY EFFECT OF INSULIN, BUT NOT OF INSULIN-LIKE GROWTH-FACTOR (IGF)-I, ON IGF BINDING-PROTEIN-1 (IGFBP-1) PRODUCTION IN HUMAN GRANULOSA-CELLS
Autore:
PORETSKY L; CHANDRASEKHER YA; BAI C; LIU HC; ROSENWAKS Z; GIUDICE L;
Indirizzi:
CORNELL UNIV,MED CTR,NEW YORK HOSP,DIV ENDOCRINOL,525 E 68TH ST NEW YORK NY 10021 CORNELL UNIV,MED CTR,NEW YORK HOSP,DEPT OBSTET & GYNECOL NEW YORK NY 10021 NEW YORK MED COLL,CABRINI MED CTR,DIV ENDOCRINOL NEW YORK NY 00000 STANFORD UNIV,MED CTR,DEPT GYNECOL & OBSTET STANFORD CA 94305
Titolo Testata:
The Journal of clinical endocrinology and metabolism
fascicolo: 2, volume: 81, anno: 1996,
pagine: 493 - 496
SICI:
0021-972X(1996)81:2<493:IMIEOI>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
FACTOR-I; LUTEAL CELLS; MONOCLONAL-ANTIBODIES; SECRETION; SUPPRESSION; MECHANISMS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
40
Recensione:
Indirizzi per estratti:
Citazione:
L. Poretsky et al., "INSULIN-RECEPTOR MEDIATES INHIBITORY EFFECT OF INSULIN, BUT NOT OF INSULIN-LIKE GROWTH-FACTOR (IGF)-I, ON IGF BINDING-PROTEIN-1 (IGFBP-1) PRODUCTION IN HUMAN GRANULOSA-CELLS", The Journal of clinical endocrinology and metabolism, 81(2), 1996, pp. 493-496

Abstract

Insulin-like growth factor binding proteins (IGFBPs) may participate in regulating ovarian function by modifying effects of insulin-like growth factors (IGFs) or by directly affecting ovarian steroidogenesis in both normal and pathological circumstances. The latter include hyperinsulinemic insulin resistant states, such as polycystic ovary syndrome. We examined regulation of IGFBP-1 production in human granulosa cells by insulin and IGF-I. The cells were obtained during in, vitro fertilization, plated in McCoy-5A tissue culture medium supplemented with 10% fetal calf serum (10(5) cells/0.5 mL), and incubated at 37 C, 90% humidity, 5% CO2 for 48 h. After additional 24 h incubation without fetal calf serum, 1, 10, or 100 ng/mL of insulin or IGF-I were added with or without 2 h preincubation with 10 mu g/mL monoclonal anti insulinreceptor antibody IR-47-9. After 48 h incubation with insulin or IGF-I, the medium was collected and IGFBP-1 and progesterone concentrations were measured, using kits from Diagnostic Systems Laboratories, Webster, TX. Progesterone concentration ranged between 50-100 ng/mL/10(5) cells, without consistent stimulatory effect of either insulin or IGF-I. Control cells produced 7.0 +/- 1.7 ng/mL of IGFBP-1. Incubation with 1 or 10 ng/mL of insulin resulted in culture medium IGFBP-1 concentrations of 7.1 +/- 1.3 ng/mL and 5.4 +/- 0.7 ng/mL, respectively (P = NS). Incubation with 100 ng/mL of insulin reduced IGFBP-1 culture medium concentration to 1.6 +/- 0.3 ng/mL, (P < 0.01, compared with controls). 1, 10, and 100 ng/mL of IGF-I inhibited IGFBP-1 concentrations in the conditioned culture medium to 1.3 +/- 0.3 ng/mL, 0.4 +/- 0.1 ng/mLand 0.3 +/- 0.1 ng/mL, respectively (P < 0.01, compared with controls). Preincubation with antiinsulin receptor antibody IR-47-9 alleviatedinhibitory effect of insulin, but not of IGF-I on IGFBP-1 production. After preincubation with IR-47-9, IGFBP-1 culture medium concentrations were 5.9 +/- 0.8 ng/mL, 4.9 +/- 1.2 ng/mL, and 4.8 +/- 1.3 ng/mL for 1, 10, and 100 ng/mL of insulin, respectively. The latter number wassignificantly higher than IGFBP-1 concentration in the medium collected from cells incubated with 100 ng/mL of insulin without IR-47-9 (1.6+/- 0.3 ng/mL, P < 0.01) and not significantly different from the control cells. For cells preincubated with IR-47-9 and then incubated with 1, 10, or 100 ng/mL of IGF-I, the IGFBP-1 conditioned culture mediumconcentrations were 1.7 +/- 0.1 ng/mL, 0.5 +/- 0.2 ng/mL, and 0.3 +/-0.1 ng/mL, respectively. None of these were significantly different from the IGFBP-1 concentrations in the medium collected from cells incubated with the respective concentrations of IGF-I without preincubation with IR-47-9. We conclude that 1) both insulin and IGF-I inhibit IGFBP-1 production by cultured human granulosa cells; 2) IGF-I is a more potent inhibitor of IGFBP-1 production than insulin; 3) in the range of hormone concentrations tested, insulin exerts its inhibitory effect on IGFBP-1 production via insulin receptor, while IGF-I appears to exert its effect via another receptor.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/12/20 alle ore 14:36:25