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Titolo:
CATHEPSIN-G AND ELASTASE IN SYNOVIAL-FLUID AND PERIPHERAL-BLOOD IN REACTIVE AND RHEUMATOID-ARTHRITIS
Autore:
NORDSTROM D; LINDY O; KONTTINEN YT; LAUHIO A; SORSA T; FRIMAN C; PETTERSSON T; SANTAVIRTA S;
Indirizzi:
HELSINKI UNIV,CENT HOSP,DIV RHEUMATOL,DEPT MED,KASARMINK 11-13 SF-00140 HELSINKI FINLAND
Titolo Testata:
Clinical rheumatology
fascicolo: 1, volume: 15, anno: 1996,
pagine: 35 - 41
SICI:
0770-3198(1996)15:1<35:CAEISA>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
NEUTROPHIL COLLAGENASE; LEUKOCYTE ELASTASE; HUMAN-MONOCYTES; CELLS; DEGRADATION; ALPHA-1-ANTITRYPSIN; PROTEINASES; LYMECYCLINE; GELATINASE; INHIBITORS;
Keywords:
NEUTROPHILIC LEUKOCYTES; SERINE PROTEINASES; SYNOVIAL FLUID; JOINT DESTRUCTION; REACTIVE ARTHRITIS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
47
Recensione:
Indirizzi per estratti:
Citazione:
D. Nordstrom et al., "CATHEPSIN-G AND ELASTASE IN SYNOVIAL-FLUID AND PERIPHERAL-BLOOD IN REACTIVE AND RHEUMATOID-ARTHRITIS", Clinical rheumatology, 15(1), 1996, pp. 35-41

Abstract

The purpose of the study was to evaluate the involvement of serine proteinases cathepsin G and elastase on pathomechanisms in synovial fluid (SF) of patients with reactive (ReA) and rheumatoid, (RA) arthritis. Cathepsin G, elastase, and their endogenous inhibitors alpha(1)-antichymotrypsin (alpha(1)-ACT) and alpha 1-proteinase inhibitor (alpha(1)-PI) were identified immunohistochemically from SF and peripheral blood(PB) of patients with ReA and RA. Cathepsin G and elastase activitiesin SF and PB were measured spectrophotometrically. Dot-immunostainingwas used to identify cathepsin G,elastase, but also alpha(1)-ACT and alpha(1)-PI from SF and PB. Cathepsin G and elastase-like activities (IU/I) were slightly elevated in ReA SF compared to the corresponding peripheral blood values (11.4 +/- 9.2 vs 4.8 +/- 1.7, NS, and 5.1 +/- 2.8 vs 2.3 +/- 2.2, NS), which was similar to what was seen in RA (16.4+/- 6.2 vs 0.53 +/- 0.4, p < 0.05, and 6.51 +/- 1.8 vs 1.22 +/- 0.58,p < 0.05). Although some samples did not contain cathepsin G and/or elastase-like activities, all samples contained immunoreactive enzyme, but also alpha(1)-ACT and alpha(1)-PI. In ReA SF, in contrast to monocytes, all polymorphonuclear (PMN) cells contained cathepsin G and elastase. Cathepsin G and elastase activities correlated,vith each other (r = 0.78, p < 0.05) suggesting PMN / primary granules as their likely source. There was a closer association between the cathepsin G or elastase and SF leukocyte count in ReA than in RA. In ReA and RA SF elevated cathepsin G and elastase activities are detected compared to activity levels in PB suggesting local production mainly from PMNs. The co-existence of highly cellular SF and cathepsin G and elastase activity in the documented presence of endogenous inhibitors in ReA SF together with the, known, usually self-remitting clinical course of ReA, suggest a brisk and even exaggerated local PMN serine proteinase release; sparing of joints does not seem to be due to lack or inhibition of PMN responses but rather to a successful down-regulation or cessation of the responses initially elicited.

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Documento generato il 20/09/20 alle ore 03:02:58